Micropipette Aspiration of Oocytes to Assess Cortical Tension.

Methods Mol Biol

Department of Cell Biology, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.

Published: March 2019

Just as it is important to understand the cell biology of signaling pathways, it is valuable also to understand mechanical forces in cells. The field of mechanobiology has a rich history, including study of cellular mechanics during mitosis and meiosis in echinoderm oocytes and zygotes dating back to the 1930s. This chapter addresses the use of micropipette aspiration (MPA) to assess cellular mechanics, specifically cortical tension, in mammalian oocytes.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158774PMC
http://dx.doi.org/10.1007/978-1-4939-8603-3_17DOI Listing

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