The Use of Biodegradable Nanoparticles for Tolerogenic Therapy of Allergic Inflammation.

Antigen-specific tolerance is the ultimate aim of treatment of allergic diseases. Here, we describe methods for the use of biodegradable nanoparticles to safely induce tolerance for the prevention and treatment of allergic inflammation in mice. Antigen is either conjugated to the surface of carboxylated poly(lactide-co-glycolide) (PLG) or encapsulated within PLG nanoparticles, and the resulting antigen-associated nanoparticles are then washed prior to intravenous injection to inhibit antigen-specific allergic immune responses.