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The Generation and Use of Allergen-Specific TCR Transgenic Animals. | LitMetric

The generation of allergen-specific TCR transgenic animals allows for the characterization of allergen-specific T-cell responses in vivo and in vitro and is a powerful tool to study adaptive immunity to allergens. Here we describe an approach starting from the isolation of antigen-specific T-cell hybridomas and using PCR, flow cytometric, and co-culture methods to obtain antigen-specific MHC class II-restricted CD4 TCR transgenic mice on the Rag2 background.

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http://dx.doi.org/10.1007/978-1-4939-7896-0_15DOI Listing

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