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Collection of optimizations for untargeted metabolomics analysis of Leishmania promastigotes using gas chromatography-mass spectrometry.
Talanta
January 2025
Center for Multiplatform Metabolomics Studies (CEMM) at the Institute of Chemistry, University of Sao Paulo, Sao Paulo, SP, 05508-000, Brazil. Electronic address:
There is no consensus in the literature regarding the ideal protocol for obtaining and preparing cell samples for untargeted metabolomics. Nevertheless, the procedures must be carefully evaluated for proper and reliable results for each organism under study. This work proposes a novel protocol for determining intracellular metabolites in Leishmania promastigotes and is fully optimized for application in conjunction with gas chromatography-mass spectrometry platforms.
View Article and Find Full Text PDFSynergistic Removal of Rare Earth Elements from Radioactive Molten Salt via Electrodeposition and Adsorption.
Inorg Chem
January 2025
Jiangxi Province Key Laboratory of Nuclear Physics and Technology, East China University of Technology, Nanchang 330013, China.
Recycling waste salt in the dry reprocessing of nuclear fuel and reducing electric energy consumption in the electrorefining process are crucial steps toward addressing significant challenges in this field. The present study proposes a novel approach to purify waste salt by selectively adsorbing excessive fission products using 5A molecular sieves (5A), based on the principles of electrorefining, with the ultimate aim of achieving sustainable development in nuclear fuel. First, Lutetium (Lu)-Bi alloy was synthesized through constant potential electrolysis in the LiCl-KCl-LuCl melt, resulting in a 90.
View Article and Find Full Text PDFRolling Bearing Fault Diagnosis Based on a Synchrosqueezing Wavelet Transform and a Transfer Residual Convolutional Neural Network.
Sensors (Basel)
January 2025
School of Information and Communication, Guilin University of Electronic Technology, Guilin 541004, China.
This study proposes a novel rolling bearing fault diagnosis technique based on a synchrosqueezing wavelet transform (SWT) and a transfer residual convolutional neural network (TRCNN) designed to address the difficulties of feature extraction caused by the non-stationarity of fault signals, as well as the issue of low fault diagnosis accuracy resulting from small sample quantities. This approach transforms the one-dimensional vibration signal into time-frequency diagrams using an SWT based on complex Morlet wavelet basis functions, which redistributes (squeezes) the values of the wavelet coefficients at different localized points in a time-frequency plane to the estimated instantaneous frequencies. This allows the energy to be more fully concentrated in actual corresponding frequency components.
View Article and Find Full Text PDFPlastic Creep Constraint in Nylon Instrument Strings.
Materials (Basel)
January 2025
Department of Engineering, University of Cambridge, Trumpington St, Cambridge CB2 1PZ, UK.
A number of rectified nylon harp strings, having the same nominal diameter, were subjected to different sequences of applied stress steps. Each string was tested continuously for several weeks to allow sufficient time for the stretching responses to be clearly observed. Qualitatively, much of the observed behaviour was in accordance with established expectations.
View Article and Find Full Text PDFEffectiveness of Mobile Health-Based Gamification Interventions for Improving Physical Activity in Individuals With Cardiovascular Diseases: Systematic Review and Meta-Analysis of Randomized Controlled Trials.
JMIR Serious Games
January 2025
School of Nursing, Jilin University, Changchun, China.
Background: Gamification refers to using game design elements in nongame contexts. Promoting physical activity (PA) through gamification is a novel and promising avenue for improving lifestyles and mitigating the advancement of cardiovascular diseases (CVDs). However, evidence of its effectiveness remains mixed.
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