Purpose: Mounting evidence highlights the essential role of in tumor initiation and malignant progression in several cancers; however, the function of in osteosarcoma (OS) remains unknown. In this study, we aim to investigate the role of and reveal its regulation by deregulated miRNAs in OS.
Materials And Methods: The expression profiles of were examined by immunohistochemistry, Western blotting, and qRT-PCR. The biological functions of were investigated through siRNA-mediated knockdown experiments. The regulation of by miR-410 was confirmed by Western blotting, dual luciferase reporter assays, and rescue experiments.
Results: was upregulated in OS tissues and cell lines, and its overexpression was positively correlated with TNM stage, metastasis, and recurrence. Knockdown of in OS cells suppressed cell proliferation, migration, invasion, and epithelial-mesenchymal transition. In addition, we identified as a novel target gene of miR-410 and miR-410 was remarkably downregulated in OS. Moreover, overexpression of miR-410 suppressed proliferation, migration, invasion, and epithelial-mesenchymal transition of OS cells by directly targeting expression. Furthermore, reintroduction of partially reversed miR-410-induced inhibitory effects on OS cells.
Conclusion: Collectively, our findings indicate that the miR-410/ link is critical in the control of OS progression.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6016597 | PMC |
| http://dx.doi.org/10.2147/OTT.S163163 | DOI Listing |
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