AI Article Synopsis

  • This study aimed to create a safe and effective treatment for oral candidiasis using a novel amplitude-modulated cold atmospheric pressure plasma jet (AM-CAPPJ) device with Helium.
  • In experiments, the AM-CAPPJ significantly reduced Candida albicans biofilm viability without harming Vero cells, showing high cell survival rates post-treatment.
  • Histological findings indicated lower inflammation in the AM-CAPPJ group compared to untreated and nystatin-treated groups, suggesting its potential for clinical use due to its dual anti-inflammatory and antifungal effects with minimal cytotoxicity.

Article Abstract

The aim of this study was to establish an effective and safe protocol for in vivo oral candidiasis treatment with atmospheric plasma jets. A novel amplitude-modulated cold atmospheric pressure plasma jet (AM-CAPPJ) device, operating with Helium, was tested. In vitro assays with Candida albicans biofilms and Vero cells were performed in order to determine the effective parameters with low cytotoxicity. After the determination of such parameters, the protocol was evaluated in experimentally induced oral candidiasis in mice. AM-CAPPJ could significantly reduce the viability of C. albicans biofilms after 5 minutes of plasma exposure when compared to the non-exposed group (p = 0.0033). After this period of exposure, high viability of Vero cells was maintained (86.33 ± 10.45%). Also, no late effects on these cells were observed after 24 and 48 hours (83.24±15.23% and 88.96±18.65%, respectively). Histological analyses revealed significantly lower occurrence of inflammatory alterations in the AM-CAPPJ group when compared to non-treated and nystatin-treated groups (p < 0.0001). Although no significant differences among the values of CFU/tongue were observed among the non-treated group and the groups treated with AM-CAPPJ or nystatin (p = 0.3201), histological analyses revealed marked reduction in candidal tissue invasion. In conclusion, these results point out to a clinical applicability of this protocol, due to the simultaneous anti-inflammatory and inhibitory effects of AM-CAPPJ with low cytotoxicity.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6021106PMC
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0199832PLOS

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