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Elucidating the Supramolecular Interaction of Positively Supercharged Fluorescent Protein with Anionic Phthalocyanines.
Adv Biol (Weinh)
October 2024
Department of Bioproducts and Biosystems, Aalto University, Aalto, 00076, Finland.
Developing bioinspired materials to convert sunlight into electricity efficiently is paramount for sustainable energy production. Fluorescent proteins are promising candidates as photoactive materials due to their high fluorescence quantum yield and absorption extinction coefficients in aqueous media. However, developing artificial bioinspired photosynthetic systems requires a detailed understanding of molecular interactions and energy transfer mechanisms in the required operating conditions.
View Article and Find Full Text PDFSupercharged coiled-coil protein with N-terminal decahistidine tag boosts siRNA complexation and delivery efficiency of a lipoproteoplex.
J Pept Sci
August 2024
Department of Chemistry, New York University, New York, New York, USA.
Short interfering RNA (siRNA) therapeutics have soared in popularity due to their highly selective and potent targeting of faulty genes, providing a non-palliative approach to address diseases. Despite their potential, effective transfection of siRNA into cells requires the assistance of an accompanying vector. Vectors constructed from non-viral materials, while offering safer and non-cytotoxic profiles, often grapple with lackluster loading and delivery efficiencies, necessitating substantial milligram quantities of expensive siRNA to confer the desired downstream effects.
View Article and Find Full Text PDFEffects of the turbocharged Miller cycle strategy on the performance improvement and emission characteristics of diesel engines.
Environ Pollut
April 2024
Department of Mechanical Science and Engineering, The University of illinois at Urbana-Champaigr, IL, 61801, USA.
Article Synopsis
- * Results indicate that the Miller cycle improves thermal efficiency in turbocharged conditions but can lower it in naturally aspirated conditions, with optimal Miller cycle ratios found to be around 30%-50%.
- * With an increase in the Miller cycle ratio, there is a significant boost in power and thermal efficiency, and under certain conditions, emissions of NOx and CO are reduced while maintaining performance.
Constructing Nucleic Acid Delivering Lipoproteoplexes from Coiled-Coil Supercharged Protein and Cationic Liposomes.
Methods Mol Biol
October 2023
Department of Chemical and Biomolecular Engineering, New York University Tandon School of Engineering, Brooklyn, NY, USA.
The safe and efficient delivery of nucleic acids is crucial for both clinical applications of gene therapy and pre-clinical laboratory research. Such delivery strategies rely on vectors to condense nucleic acid payloads and escort them into the cell without being degraded in the extracellular environment; however, the construction and utilization of these vectors can be difficult and time-consuming. Here, we detail the steps involved in the rapid, laboratory-scale production and assessment of a versatile, nucleic acid delivery vehicle, known as the lipoproteoplex.
View Article and Find Full Text PDFTop-Down Analysis of Supercharged Proteins Using Collision-, Electron-, and Photon-Based Activation Methods.
J Am Soc Mass Spectrom
July 2023
Department of Chemistry, University of Texas at Austin, Austin, Texas 78712, United States.
The impact of supercharging on the fragmentation patterns of six proteins, ubiquitin, cytochrome c, staph nuclease, myoglobin, dihydrofolate reductase, and carbonic anhydrase, was investigated for five activation methods, HCD, ETD, EThcD, 213 nm UVPD, and 193 nm UVPD under denaturing conditions. Changes in sequence coverage, alterations in the number and abundance of preferential cleavages (N-terminal to proline, C-terminal to aspartic or glutamic acid, adjacent to aromatic residues), and changes in individual fragment ion abundances were evaluated. Large decreases in sequence coverage were observed upon supercharging of proteins activated by HCD, whereas modest gains were observed for ETD.
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