Background: Mitogen-activated protein kinase (MAPK) cascades, an important type of pathway in eukaryotic signaling networks, play a key role in plant defense responses, growth and development.
Methods: Phylogenetic analysis and conserved motif analysis of the MKK and MPK families in , and classified genes and genes. qRT-PCR was used for the expression patterns of and genes, and yeast two-hybrid assay was applied to clear the interaction between CmMPKs and CmMKKs.
Results: We characterized six genes and 11 genes in chrysanthemum based on transcriptomic sequences and classified these genes into four groups. qRT-PCR analysis demonstrated that s and s exhibited various expression patterns in different organs of chrysanthemum and in response to abiotic stresses and phytohormone treatments. Furthermore, a yeast two-hybrid assay was applied to analyze the interaction between CmMKKs and CmMPKs and reveal the MAPK cascades in chrysanthemum.
Discussion: Our data led us to propose that CmMKK4-CmMPK13 and CmMKK2-CmMPK4 may be involved in regulating salt resistance and in the relationship between CmMKK9 and CmMPK6 and temperature stress.
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| http://dx.doi.org/10.7717/peerj.5037 | DOI Listing |
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