AI Article Synopsis

  • Sirt1 is a member of the Sirtuin family that plays a crucial role in growth, metabolism, and lifespan, as well as regulating the circadian clock in relation to feeding.
  • A study found that Sirt1 is essential for food anticipatory activity (FAA), with Sirt1 overexpressing mice showing enhanced FAA, while Sirt1-lacking mice showed minimal FAA.
  • Despite various methods of inactivating Sirt1 in mice, the research concluded that FAA operates independently of Sirt1, challenging previous assumptions about its role.

Article Abstract

A number of studies have demonstrated that the Sirtuin family member, Sirt1, is a key integrator of growth, metabolism, and lifespan. Sirt1 directly interacts with and deacetylates key regulators of the circadian clock, positioning it to be an important link between feeding and circadian rhythms. In fact, one study suggests that Sirt1 is necessary for behavioral anticipation of limited daily food availability, a circadian process termed food anticipatory activity (FAA). In their study, mice overexpressing Sirt1 had enhanced FAA, while mice lacking Sirt1 had little to no FAA. Based on the supposition that Sirt1 was indeed required for FAA, we sought to use Sirt1 deletion to map the neural circuitry responsible for FAA. We began by inactivating Sirt1 using the cell-type specific Cre-driver lines proopiomelanocortin, but after observing no effect on body weight loss or FAA we then moved on to more broadly neuronal Cre drivers Ca2+/calmodulin-dependent protein kinase II and nestin. As neither of these neuronal deletions of Sirt1 had impaired FAA, we then tested 1) a broad postnatal tamoxifen-inducible deletion, 2) a complete, developmental knockout of Sirt1, and 3) a gene replacement, catalytically inactive, form of Sirt1; but all of these mice had FAA similar to controls. Therefore, our findings suggest that FAA is completely independent of Sirt1.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6016907PMC
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0199586PLOS

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