The paper covers experimental results of introducing exogenic genetic material, namely DNA sequences of the Rous sarcoma virus, by microinjections in mice zygotes and Drosophila early embryos. In a number of cases integration of viral DNA into genomes of these organisms was detected. Blot-hybridizations analysis of cell DNA proved that the inserted viral sequences undergo rearrangements in the course of integration.
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Bioinformatics
January 2025
Institute for Computational Systems Biology, Universität Hamburg, Hamburg, 22761, Germany.
Motivation: Transcription factors (TFs) are DNA-binding proteins that regulate gene expression. Traditional methods predict a protein as a TF if the protein contains any DNA-binding domains (DBDs) of known TFs. However, this approach fails to identify a novel TF that does not contain any known DBDs.
View Article and Find Full Text PDFBackground: The outcomes of extensive Genome-Wide Association Study (GWAS) data in Polygenic Risk Score (PRS) studies exhibit varying odds ratios (ORs), ranging from 1.13 (Bellenguez) to 1.34 (Kunkle).
View Article and Find Full Text PDFAlzheimers Dement
December 2024
Cleveland Clinic Lou Ruvo Center for Brain Health, Cleveland, OH, USA.
Background: Inflammatory changes are a key element of Alzheimer's disease (AD)pathophysiology. However, there is lack of clarity regarding key gene drivers of the cytokines identified as playing a role in clinical AD progression and their relationship to AD biomarkers to understand their clinical role.
Method: We investigated transcriptomic datasets from Alzheimer's Disease Neuroimaging Initiative (ADNI) and Harvard Brain Tissue Resource Center (HBTRC) to evaluate regulatory gene networks in human AD.
Alzheimers Dement
December 2024
Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA.
Background: Not all people with neuropathological evidence of Alzheimer's disease (AD) manifest clinical symptoms in life (cognitive resilience). We aimed to identify genetic and epigenetic signatures of cognitive resilience, utilizing data from brain donors with neuropathological evidence of AD who were either symptomatic or asymptomatic in life.
Method: Among brain donors with neuropathologically-confirmed AD (364 asymptomatic/cognitively resilient and 490 symptomatic) from the Boston University AD Research Center, Framingham Heart Study-where we generated our own data-as well as the Religious Orders Study and Rush Memory and Aging Project, we utilized genome-wide genetic array data, genome-wide DNA methylation array data and RNA sequencing data.
Alzheimers Dement
December 2024
Brain Research Institute, Niigata University, Niigata, Niigata, Japan.
Background: The Alzheimer's disease treatment drug "Lecanemab" has been approved in the US, Japan, and China, and administration has begun in clinical treatment. Other anti-Aβ antibody drugs (e.g.
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