AI Article Synopsis
- Entolimod, a flagellin derivative, has shown effectiveness in protecting animals from radiation but causes flu-like symptoms, indicating potential toxicity and immunogenicity.
- Researchers successfully created a cyclic form of entolimod using a split DnaE intein method, achieving nearly 100% cyclization efficiency and employed various purification strategies due to the different structures of the linear and circular forms.
- The cyclic entolimod demonstrated significantly enhanced NF-κB signaling and improved radioprotective effects in mice, suggesting its potential for future clinical studies.
Article Abstract
Background: Entolimod is a flagellin derivate. Previous work has demonstrated that entolimod effectively protects mice and non-human primates from ionizing radiation. However, it caused a "flu-like" syndrome after radioprotective and anticancer clinical application, indicating some type of immunogenicity and toxicity. Cyclization is commonly used to improve the in vivo stability and activity of peptides and proteins.
Methods: We designed and constructed cyclic entolimod using split DnaE intein with almost 100% cyclization efficiency. We adopted different strategies to purify the linear and circular entolimod due to their different topologies. Both of linear and circular entolimod were first purified by Ni-chelating affinity chromatography, and then the linear and circular entolimod were purified by size-exclusion and ion-exchange chromatography, respectively.
Results: The circular entolimod showed significantly increased both the in vitro NF-κB signaling and in vivo radioprotective activity in mice.
Conclusion: Our data indicates that circular entolimod might be a good candidate for further clinical investigation.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6011820 | PMC |
| http://dx.doi.org/10.7717/peerj.5043 | DOI Listing |
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