AI Article Synopsis
- The binary system uses HK022 integrase to activate a silenced Diphtheria toxin gene specifically in cancer cells, controlled by a cancer-specific promoter.
- In a lung cancer mouse model, this system showed higher specificity and efficiency compared to a traditional mono system that directly expresses the toxin.
- Mice treated with the binary system had longer survival rates than those treated with the mono system or left untreated, likely due to the overexpression of suppressing factors in the mono system.
Article Abstract
The binary system presented in this work is based on the bacteriophage HK022 integrase recombinase that activates the expression of a silenced Diphtheria toxin gene, both controlled by the cancer specific promoter. Using a lung cancer mice model, assays of different apoptotic and anti-apoptotic factors have demonstrated that the Integrase based binary system is highly specific towards cancer cells and more efficient compared to the conventional mono system whose toxin is directly expressed under . In a mice survival test, this binary system demonstrated longer persistence compared to the untreated and the mono treated ones. The reason underlying the advantage of this binary system over the mono system seems to be an overexpression of various suppressing factors induced by the mono system.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6007955 | PMC |
| http://dx.doi.org/10.18632/oncotarget.25512 | DOI Listing |
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