Background: Streptococcus suis (SS) is a major swine pathogen and a serious zoonotic pathogen causing septicemia and meningitis in piglets and humans. Using an immunoproteomic approach, we previously brought evidence that ornithine carbamoytransferase (OCT) may represent a vaccine candidate to protect against S. suis biofilm-related and acute infections.
Method: In this study, the gene encoding OCT was cloned into the expression vector pET-28a and the recombinant protein was expressed in Escherichia coli BL21. The immunogenicity and protective efficacy of the SS OCT was further investigated in a mouse model.
Results: The protein was found to be expressed in vivo and elicited high antibody titers following SS infections in mice. An animal challenge experiment with SS showed that 62.5% of mice immunized with the OCT protein were protected. Using an in vitro competitive adherence inhibition assay of adherence, evidence was obtained that OCT could significantly reduce the number of SS cells adhered to porcine kidney PK-15 cells. The bacterial levels recovered in mice of the OCT immunized group were significantly decreased in some organs, compared with the control group.
Conclusion: In summary, our results suggest that the recombinant SS OCT protein, which is involved in bacterial adherence, may efficiently stimulate an immune response conferring protection against SS infections. It may therefore be considered as a potential vaccine candidate, although further studies are necessary to evaluate their use in swine.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jmii.2018.05.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A surface protein identified from Streptococcus suis serotype 2 exhibits neutrophil-resistant ability via its polysaccharide capsule.
Microb Pathog
December 2024
MOE Joint International Research Laboratory of Animal Health and Food Safety, Nanjing Agricultural University, Nanjing 210095, PR China; College of Animal Science, Anhui Science and Technology University, Fengyang, 233100, PR China. Electronic address:
Article Synopsis
- Streptococcus suis serotype 2 (SS2) is a zoonotic bacteria that can cause serious diseases, but how it avoids the body's immune system is not well understood.
- A study identified a specific protein mutation in SS2 that leads to increased neutrophil responses, yet the mutated bacteria survive poorly in the bloodstream compared to the original strain.
- The deletion of this protein results in a weaker protective capsule and boosts the bacteria's ability to form biofilms; however, it also decreases the bacteria's survival within immune cells, highlighting potential targets for preventing infections.
First case of Streptococcus suis bacteremia caused by a serotype 14 strain without pig or pork contact in the Netherlands: A case report.
Diagn Microbiol Infect Dis
December 2024
Amsterdam UMC location University of Amsterdam, Department of Global Health, Amsterdam Institute for Global Health and Development, Paasheuvelweg 25, Amsterdam, the Netherlands; Amsterdam UMC location University of Amsterdam, Department of Medical Microbiology and Infection Prevention, Meibergdreef 9, Amsterdam, the Netherlands.
Streptococcus suis is a porcine pathogen that causes severe zoonotic infections in humans resulting in meningitis and sepsis. The main risk factors for S. suis zoonotic infections are consumption of raw pork products and direct contact with live pigs or pork, in particular in the presence of skin injuries.
View Article and Find Full Text PDFGenomic analysis and virulence of human Streptococcus suis serotype 14.
Eur J Clin Microbiol Infect Dis
December 2024
Faculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand.
Purpose: Streptococcus suis serotype 14 is the second most prevalent serotype being highly prevalent in Southeast Asia. This study aimed to characterize genetic background, population structure, virulent genes, antimicrobial-resistant genes, and virulence of human S. suis serotype 14.
View Article and Find Full Text PDFThree New Dipeptide and Two New Polyketide Derivatives from the Mangrove-Derived Fungus sp.: Antioxidant Activity of Two Isolated Substances.
Mar Drugs
December 2024
CAS Key Laboratory of Tropical Marine Bio-resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
Five new metabolites, including three cyclic dipeptide derivatives (-) and two new polyketides (-), together with nine known ones (- and -), were isolated from the mangrove-sediments-derived fungus sp. SCSIO 41431. Their structures were determined using detailed NMR, MS spectroscopic analyses, and quantum chemical calculations.
View Article and Find Full Text PDFCharacterization of integrative and conjugative elements carrying erm(B) and tet(O) resistance determinants in streptococcus uberis isolates from bovine milk in Chiba prefecture, Japan: CompArative GEne cluster analysis toolbox with ICEfinder.
BMC Res Notes
December 2024
Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences and Ōmura Satoshi Memorial Institute, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, 108-8641, Japan.
Objective: We aimed to characterize integrative and conjugative elements (ICEs) in antimicrobial resistant Streptococcs uberis isolates from bovine milk in Chiba, Japan, based on whole-genome sequence (WGS) data.
Results: Of the 101 isolates, we found the 36 isolates harboring erm(B)-tet(O), showing resistance to macrolides-lincosamides-tetracyclines. The 22 isolates were randomly selected and subject to WGS determination.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!