AI Article Synopsis
- The study investigates how proteins of various sizes are released from cellulose sulphate capsules, highlighting that proteins up to around 70kD can be effectively released.
- The release of proteins is influenced by factors such as their conformation, charge, and concentration, with smaller proteins like cytokines being particularly easy to release.
- The findings suggest that using encapsulated, genetically engineered cells to produce cytokines like IL-2 could enhance cancer immunotherapies and potentially allow for the use of donor cells in treatment, bolstered by cellulose sulphate's proven safety in patients.
Article Abstract
The size and speed of release of proteins of different sizes from standard cellulose sulphate capsules (Cell-in-a-Box®) was investigated. Proteins with molecular weights of up to around 70kD can be released. The conformation, charge and concentration of the protein being released play a role in the release kinetics. Small proteins such as cytokines can be easily released. The ability to produce cytokines at a sustained and predefined level from encapsulated cells genetically engineered to overexpress such cytokines and implanted into patients may aid immunotherapies of cancer as well as infectious and other diseases. It will also allow allogeneic rather than autologous cells to be used. We show that cells encapsulated in polymers of cellulose sulphate are able to release cytokines such as interleukin-2 (IL-2) in a stimulated fashion e.g. using phorbol 12-myristate 13-acetate (PMA) plus ionomycin. Given the excellent documented safety record of cellulose sulphate in patients, these data suggest that clinical usage of the technology may be warranted for cancer treatment and other diseases.
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| http://dx.doi.org/10.1016/j.ijpharm.2018.06.040 | DOI Listing |
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Article Synopsis
- Two phosphorus-based flame retardants, APTA and DUPT, were synthesized and characterized using NMR and FTIR techniques.
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