[Effects of fasudil on isoproterenol induced cardiac hypertrophy in rats].

Zhongguo Ying Yong Sheng Li Xue Za Zhi

College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China.

Published: May 2016

AI Article Synopsis

  • The study aimed to explore how fasudil (Fas) affects cardiac hypertrophy (CH) in rats induced by isoprenaline (Iso).
  • Rats were divided into four groups, with one receiving Iso to induce CH and others treated with varying doses of Fas for 8 weeks.
  • Results showed that Fas treatment improved heart function and reduced cellular changes associated with CH, suggesting it may have protective effects on the heart by lowering harmful protein expressions and improving tissue damage.

Article Abstract

Objective: To investigate the effects and mechanisms of fasudil (Fas) on cardiac hypertrophy (CH) induced by isoprenaline(Iso) in rats.

Methods: Except for the normal control group, the rest three groups rats were injected subcutaneously with Iso(5mg/kg) for setting up CH model. SD rats were randomly divided into four groups:Normal control group, Iso model group, Fas with low-dose group (5 mg/kg, i.p) and Fas with high-dose group (20 mg/kg, i.p). Animals were treated with Fas for 8 weeks. After the treatment, the following index were detected:heart rate (HR), left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP), left ventricular systolic maximum rate (+dp/dt) and left ventricular diastolic maximum rate (-dp/dt). The body weight(BW) and heart weight (HW) were weighed and heart weight index (HWI) was calculateed. Myocardial tissue specimens, HE and Masson staining were performed for observing the histopathological changes. The protein expression of extracellular signal-regulated kinase 1/2 (ERK1/2) in myocardial tissue was determined by using immunohistochemical methods. The expression of ERK1/2 mRNA in the myocardial tissue was detected by using semi-quantitative RT-PCR method.

Results: Compared with normal control group, HR and LVEDP wereincreased significantly, while LVSP and ±dp/dtmax were decreased significantly; HWI was increased significantly in CH group; The myocardial cell volume and the cell gap were increased,cells arranged disorder, and severe myocardial interstitial fibrosis was presented in CH group. The expression of ERK1/2 mRNA was significantly increased. After Fas treatment, the systolic and diastolic functions of heart were improved, cell volume was reduced, cell gap became small, cells arranged in neat rows, and the fibrosis was significantly reduced. The expressions of RhoA, ERK1/2 mRNA were significantly reduced. The damages of myocardial tissue were improved in different degrees.

Conclusions: ERK1/2 signaling pathway is involved in Iso induced CH. Fas protects against the CH of rats induced by Iso, which mechanisms may be related with blocking ERK1/2 signaling pathway.

Download full-text PDF

Source
http://dx.doi.org/10.13459/j.cnki.cjap.2016.05.008DOI Listing

Publication Analysis

Top Keywords

left ventricular
16
myocardial tissue
16
control group
12
erk1/2 mrna
12
cardiac hypertrophy
8
normal control
8
group
8
group mg/kg
8
ventricular systolic
8
ventricular diastolic
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!