AI Article Synopsis
- Bacillus sp. SJ-10 is a halotolerant bacterium isolated from salt-fermented fish, known for its ability to thrive and metabolize in high salt environments.
- The complete genome of Bacillus sp. SJ-10 is 4,041,649 base pairs long, featuring a single circular chromosome and a guanine-cytosine content of 46.39%.
- This bacterium produces a significant enzyme complex, including γ-PGA synthases and various salt-stable enzymes useful in multiple industries such as food, beverages, and detergents, offering insights into the survival strategies of halotolerant bacteria.
Article Abstract
Bacillus sp. SJ-10 (KCCM 90078, JCM 15709) is a halotolerant bacterium isolated from a traditional Korean food, i.e., salt-fermented fish (jeotgal). The bacterium can survive and engage in metabolism at high salt concentrations. Here, we reported complete genome sequence of Bacillus sp. SJ-10, which has a single circular chromosome of 4,041,649 base pairs with a guanine-cytosine content of 46.39%. Bacillus sp. SJ-10 encodes a subunit of poly-γ-glutamic acid (γ-PGA) with a molecular weight of approximately 400 kDa, which contains four γ-PGA synthases (pgsB, pgsC, pgsAA and pgsE) and one γ-PGA-releasing gene (pgsS). This bacterium also able to produce salt-stable enzymes such as protease, β-glucosidase, and β-1,3-1,4-glucanase. This affords significant insights into strategies employed by halotolerant bacteria to survive at high salt concentrations. The sequence contains information on secondary metabolites biosynthetic gene cluster, and most importantly enzymes produced by the bacterium may be valuable with respect to food, beverage, detergent, animal feed, and certain commercial contexts.
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| http://dx.doi.org/10.1007/s00284-018-1533-x | DOI Listing |
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