Profiling of the Transcriptomic Responses of Upon Treatment With Secretome.

strain ACM941 is a fungal bio-control agent patented against the causative agent of Fusarium Head Blight, . Although the molecular details remain enigmatic, previous studies have suggested that may secrete growth inhibitors. Further toward this, experiments described herein show that induction of cultures by the addition of an aliquot of -spent media (including macroconidia), yield -spent media that elicited higher anti- activity than either control or deoxynivalenol (DON)-induced -spent media. To gain additional insight into the genetic and metabolic factors modulating this interaction, transcriptomic (RNAseq) profiles of in response to DON and spent media treatment, were developed. This analysis revealed 24,112 unigenes, of which 5,605 and 6,285 were differentially regulated by DON and spent media, respectively. More than half of these unigenes were up-regulated, with annotations, most notably in the -spent media treatment data, suggesting enhancement of polyketide (PK) and non-ribosomal peptide (NRP) secondary metabolite precursor synthesis, and PK/NRP-like synthases. Four ABC transporters were also up-regulated in response to spent media. Further analysis showed that the PK and NRP-like synthases belong to three gene clusters that also include ABC transporters, and other genes known to tailor secondary metabolite biosynthesis. The RNAseq data was further validated using quantitative RT-qPCR. Taken together, these results show that responds to the presence of -spent media (and to a lesser extent, DON-alone) by up-regulating unique aspects of its secondary metabolism-related genetic repertoire. The identities and roles of secondary metabolites produced by the targeted gene clusters are now under investigation.