Objective: To explore whether targeting phosphoglycerate kinase 1 (PGK1) can enhance the sensitivity of BRAF mutation melanoma cells to vemurafenib.
Methods: The methods of cell biology, molecular biology and pharmacology(MTT assay, Western blot, FCM, Colongenic assay) were used in this study.
Results: ① Silencing of PGK1 expression increased the efficacy of vemurafenib in melanoma cells, as evidenced by greater killing in the tumor cells subjected to combined treatment of vemurafenib with siPGK1; ②The mechanism of enhanced sensitivity of melanoma cells to vemurafenib was associated with activation of apoptotic signaling pathway.
Conclusions: Targeting of PGK1 may represent a novel strategy of sensitizing melanoma cells to vemurafinib.
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| http://dx.doi.org/10.12047/j.cjap.5559.2017.071 | DOI Listing |
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