Regeneration of tissue, including bone, using mesenchymal stem cells (MSCs) has been progressing rapidly. Regeneration of bone requires the presence of an appropriate environment and efficient chemotaxis of cells to the target site. Differentiation of MSCs into mesenchymal cells has received considerable attention, but the effect of MSCs on chemotaxis is not well understood. In this study, we investigated the effect of MSCs on chemotaxis of RAW264 cells via C-C motif chemokine ligand 2 (CCL2). Balb/c mouse bone marrow-derived MSCs and RAW264 cells, which are osteoclast precursor cells, were co-cultured without cell contact. The gene expression of CCL2 in MSCs and CC-chemokine receptor 2 (CCR2) in RAW264 cells was determined using quantitative real-time PCR. Analysis of RAW264 cell chemotaxis was performed using the Boyden chamber assay. mRNAs for CCL2 and CCR2 were significantly upregulated upon co-culture in comparison to culture of either cell type alone, and the number of chemotactic RAW264 cells was significantly increased by co-culture. MSCs enhanced the chemotaxis of RAW264 cells, possibly via CCL2-CCR2 interaction, suggesting the potential utility of MSCs for tissue regeneration.

Download full-text PDF

Source
http://dx.doi.org/10.2334/josnusd.17-0187DOI Listing

Publication Analysis

Top Keywords

raw264 cells
20
cells
11
mesenchymal stem
8
stem cells
8
osteoclast precursor
8
precursor cells
8
mscs
8
mscs chemotaxis
8
chemotaxis raw264
8
chemotaxis
6

Similar Publications

Selective serotonin reuptake inhibitor correlates with decreased bone mineral density and impedes orthodontic tooth movement. The present study aimed to examine the effects of fluoxetine on osteoclast differentiation and function. Human peripheral blood mononuclear cells (hPBMCs) and murine RAW264.

View Article and Find Full Text PDF

Structural analysis and immunological activity of a novel low molecular weight neutral polysaccharide isolated from Hemerocallis citrina Borani.

Food Chem

December 2024

State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address:

A novel neutral water-soluble polysaccharide (HCBP1-1) was isolated and purified from Hemerocallis citrina Borani by column chromatography. The fine structure of HCBP1-1 was determined by a series of physical and chemical means. HCBP1-1 was a homogeneous low molecular weight polysaccharide of 7.

View Article and Find Full Text PDF

Protective effect of Astragaloside II against lung injury in COPD based on mTORC1/GSK-3β signaling pathway.

Eur J Pharmacol

December 2024

Department of Respiratory and Critical Care Medicine, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China. Electronic address:

Background: Astragaloside II (AST II) is one of the principal bioactive components of Astragalus mongholicus Bunge, exhibiting multiple pharmacological properties. However, the therapeutic efficacy of AST II in Chronic Obstructive Pulmonary Disease (COPD) remains to be fully elucidated. The study explored the effects and mechanisms of AST II in a COPD model induced by exposure to cigarette smoke (CS) and lipopolysaccharide (LPS) in mice.

View Article and Find Full Text PDF

Physicochemical characteristics and anti-inflammatory properties of Zophobas morio (super mealworm) protein extracted by different methods.

Food Chem

December 2024

School of Food Science and Technology, Kyungpook National University, Daegu 702-701, Republic of Korea; Research Institute of Tailored Food Technology, Kyungpook National University, Daegu 41566, Republic of Korea. Electronic address:

In this study, Zophobas morio protein (ZMP) was extracted via combining alkaline extraction with ultrasound-assisted (AE-UAE) or microwave-assisted (AE-MAE) extraction in comparison with their respective single extraction methods and conventional method. AE-UAE and AE-MAE exhibited a higher extraction yield (40.68 % and 36.

View Article and Find Full Text PDF

Jujube ( Mill.) has been consumed globally as a fruit and a nutraceutical food for millennia. This study presents the isolation and purification of a novel water-soluble polysaccharide fraction, ZJMP-2, from Mill.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!