AI Article Synopsis
- The study highlights that testing for the HLA-A*31:01 gene can help predict hypersensitivity reactions to the medication carbamazepine, and it's recommended to conduct this test before starting the drug.
- A new real-time PCR test was developed specifically for detecting the HLA-A*31:01 allele, showing 100% agreement with a more traditional sequencing method in a test group of 100 individuals.
- The results reveal high reliability for this test, with perfect sensitivity and specificity, and varying carrier rates of HLA-A*31:01 among different ethnic groups, suggesting its importance in preventing adverse drug reactions.
Article Abstract
Aim: HLA-A*31:01 has been associated with carbamazepine-induced hypersensitivity reactions. HLA-A*31:01 genetic testing is recommended before the initiation of carbamazepine therapy.
Methods: A novel real-time PCR assay was designed for HLA-A*31:01 detection by allele-specific primers and TaqMan minor groove binding probes.
Results: The genotyping results in 100 subjects by the established method who were in 100% agreement with the sequencing-based typing method. The assay presents a sensitivity of 1 (95% CI: 0.69-1.00), a specificity of 1 (95% CI: 0.96-1.00) and a positive and negative predictive value of 1. The carrier rates of HLA-A*31:01 in Tibetan (n = 45), Han Chinese (n = 100), Miaos (n = 48) and Khalkhas (n = 48) were 22.2, 10, 4.2 and 18.8%, respectively.
Conclusion: This assay is reliable to detect HLA-A*31:01 and would be useful to prevent carbamazepine-induced hypersensitivity reactions.
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Source |
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| http://dx.doi.org/10.2217/pgs-2018-0050 | DOI Listing |
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