AI Article Synopsis
- The study examined how the triazole fungicide tebuconazole affects male rats by inducing cytochrome P450 enzymes, causing oxidative stress, and disrupting endocrine function.
- Tebuconazole increased various liver enzymes linked to drug metabolism while decreasing enzyme activity in the testes, showcasing its complex impact on organ functionality.
- Additionally, the treatment resulted in lower testosterone levels and sperm count, indicating potential anti-androgenic effects.
Article Abstract
The major objective of the present study was to determine the ability of a triazole fungicide tebuconazole to induce cytochrome P450-dependent monooxygenases, oxidative stress, and endocrine-disrupting activity using male rats treated with tebuconazole at 10, 25, and 50 mg/kg p.o. once daily for 28 days. In liver, tebuconazole dose-dependently increased microsomal contents of cytochrome P450 and cytochrome b and the activities of NADPH-cytochrome P450 reductase, 7-ethoxyresorufin O-deethylase, methoxyresorufin O-demethylase, pentoxyresorufin O-dealkylase, 7-ethoxycoumarin O-deethylase, aniline hydroxylase, and erythromycin N-demethylase. In kidney, tebuconazole increased 7-ethoxycoumarin O-deethylase activity without affecting other monooxygenase activities. In marked contrast to liver and kidney, tebuconazole decreased testicular 7-ethoxyresorufin O-deethylase, methoxyresorufin O-demethylase, 7-ethoxycoumarin O-deethylase, aniline hydroxylase, and erythromycin N-demethylase activities. The results of immunoblot analysis of liver microsomes of controls and tebuconazole-treated rats revealed that tebuconazole induced CYP1A1/2, CYP2B1/2, CYP2E1, and CYP3A proteins in liver. Additions of tebuconazole to liver microsomes inhibited microsomal 7-ethoxycoumarin O-deethylase activity in vitro (IC = 1.50-1.69 µM). Treatment of rats with tebuconazole decreased glutathione content and increased glutathione S-transferase, superoxide dismutase, catalase, and glutathione peroxidase activities in liver; increased superoxide dismutase activities in kidney and testis; but decreased glutathione S-transferase activity in testis. Treatments with tebuconazole decreased serum testosterone concentration and cauda epididymal sperm count. The present study demonstrates that tebuconazole induces a multiplicity of CYPs and oxidative stress in liver; inhibits testicular P450 and glutathione S-transferase activities; and produces anti-androgenic effects in male rats.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/tox.22575 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Optimization of assay conditions to quantify ECOD activity in vivo in individual Daphnia magna. Assay performance evaluation with model CYP 450 inducers/inhibitors.
Ecotoxicol Environ Saf
March 2024
Balaton Limnological Research Institute, Hungarian Research Network, Klebelsberg Kuno u. 3., Tihany H-8237, Hungary.
Screening the activity of the cytochrome P450 (CYP450) mixed function oxidase system in aquatic invertebrates received seldom applications in ecotoxicology due to low baseline enzymatic activities characteristic for these organisms. In this study, an existing in vivo spectrofluorometric assay method based on quantifying the cytochrome P450 mediated conversion of 7-ethocycoumarin (EtC) used as substrate to the product 7-hydroxycoumarin (HCm) called: ethoxycoumarin-O-deethylase (ECOD) activity, initially applicable on pooled samples of Daphnia magna, was optimized for use on individual organisms. Optimal assay conditions have been established for as small as 3- and 6 days old individuals, and the limits of spectrofluorometric detection of HCm excreted by daphnids in the incubation media were defined.
View Article and Find Full Text PDFToxicity and Mechanisms of Action of Polycyclic Aromatic Hydrocarbon Pollution in Red Algae (Gracilaria corticata) from the Northern Coast of the Oman Sea.
Environ Toxicol Chem
September 2019
Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran.
The activities of selected biomarkers including 7-ethoxycoumarin-O-deethylase (ECOD) and glutathione S-transferase (GST) were measured in the red algae (Gracilaria corticata) obtained from the Oman Sea. Chemical analyses were used to assess the polycyclic aromatic hydrocarbons (PAHs) in the red algae. Total PAH concentrations in the red algae ranged from 3.
View Article and Find Full Text PDFKnock-Down of Gossypol-Inducing Cytochrome P450 Genes Reduced Deltamethrin Sensitivity in (Hübner).
Int J Mol Sci
May 2019
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
Plants employ an intricate and dynamic defense system that includes physiological, biochemical, and molecular mechanisms to counteract the effects of herbivorous attacks. In addition to their tolerance to phytotoxins, beet armyworm has quickly developed resistance to deltamethrin; a widely used pyrethroid insecticide in cotton fields. The lethal concentration (LC) required to kill 50% of the population of deltamethrin to gossypol-fed larvae was 2.
View Article and Find Full Text PDFEffects of Field-Realistic Concentrations of Carbendazim on Survival and Physiology in Forager Honey Bees (Hymenoptera: Apidae).
J Insect Sci
July 2018
School of Plant Protection, Anhui Agricultural University, Hefei, China.
Carbendazim is nowadays widely used to control fungus in various nectariferous crops. Little is known about how honey bees, Apis mellifera L. (Hymenoptera: Apidae), respond to carbendazim exposure.
View Article and Find Full Text PDFAcute toxicity and sublethal effects of myclobutanil on respiration, flight and detoxification enzymes in Apis cerana cerana.
Pestic Biochem Physiol
May 2018
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; Bee Industry Technology Research Center, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101,China. Electronic address:
Myclobutanil is currently used on the flowering plants. Little is known about how Apis cerana cerana respond to myclobutanil exposure. Hence, the acute toxicity of myclobutanil and its sublethal effects on respiration, flight and detoxification enzymes [7-ethoxycoumarin O-deethylase (ECOD) and glutathione S-transferases (GSTs)] in A.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!