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Optimization of subculture and DNA extraction steps within the whole genome sequencing workflow for source tracking of Salmonella enterica and Listeria monocytogenes. | LitMetric
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Optimization of subculture and DNA extraction steps within the whole genome sequencing workflow for source tracking of Salmonella enterica and Listeria monocytogenes.

Johan Gimonet Anne-Catherine Portmann Coralie Fournier Leen Baert

J Microbiol Methods

Nestlé Research Center, Nestec Ltd, Vers-Chez-les-Blanc, 1000 Lausanne 26, Switzerland. Electronic address:

Published: August 2018

This work shows that an incubation time reduced to 4-5 h to prepare a culture for DNA extraction followed by an automated DNA extraction can shorten the hands-on time, the turnaround time by 30% and increase the throughput while maintaining the WGS quality assessed by high quality Single Nucleotide Polymorphism analysis.

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http://dx.doi.org/10.1016/j.mimet.2018.06.005DOI Listing

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