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Plant cross-fertilization for production of dual-specific antibodies targeting both Ebola virus-like particles and HER2 protein in F plants.
Genes Genomics
January 2025
Department of Medicine, BioSystems Design Lab, College of Medicine, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul, 06974, Korea.
Background: This study explores the cross-fertilization of transgenic tobacco plants to produce dual-specific monoclonal antibodies (mAbs) targeting Ebola virus-like particles and HER2 proteins. We generated F plants by hybridizing individual transgenic lines expressing the anti-HER2 breast cancer VHH mAb (HV) and the H-13F6 human anti-Ebola large single chain mAb (EL).
Objective: Hybridizing transgenic plants to express dual-antibodies between different structures VHH and LSCK indicate the potential of transgenic plants as a cost-effective and scalable production system for dual targeting mAbs.
The bidirectional relationship between pain and tobacco use: Insights from the longitudinal Population Assessment of Tobacco and Health (PATH) study.
Drug Alcohol Depend
January 2025
Department of Psychiatry, Yale University School of Medicine, New Haven, CT, United States; VA Connecticut Healthcare System, West Haven, CT, United States; Clinical Neuroscience Research Unit (CNRU), Connecticut Mental Health Center (CMHC), New Haven, CT, United States. Electronic address:
Background: Tobacco smoking remains the leading preventable cause of death, whereas chronic pain is the leading cause of disability. Chronic pain and tobacco smoking are closely interrelated. We investigated whether pain predicts daily cigarette smoking and if daily cigarette smoking predicts the development of pain.
View Article and Find Full Text PDFThe psychometric properties of a new scale of dehumanizing deindividuation in couples.
J Fam Psychol
January 2025
Department of Psychology, University of Nebraska-Lincoln.
Emerging research indicates that dehumanization may occur in couples with serious consequences; however, this research is in its infancy, and there is a need to integrate dehumanization perspectives with key theories of intimate relationships to best understand this phenomenon. Drawing on work on individuation in couples, we present an integrated framework of dehumanizing deindividuation that is characterized by derogation (viewing a partner with contempt), disregard (ignoring or overlooking a partner), and denial of autonomy (restricting a partner's self-determination). We present data from two samples highlighting the reliability and validity of a new measure, the Dehumanizing Deindividuation in Couples (DDC) scale, which was internally consistent and had excellent construct replicability.
View Article and Find Full Text PDFIntegrated metabolomics and transcriptomics reveal the role of calcium sugar alcohol in the regulation of phenolic acid biosynthesis in Torreya grandis nuts.
BMC Plant Biol
January 2025
State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, People's Republic of China.
Background: Torreya grandis, a prominent tree species of the autochthonous subtropical region of China, possesses a drupe-like fruit containing a nut that is rich in nutrients and bioactive compounds. However, the effect of calcium (Ca) sugar alcohol (CSA), a newly developed chelated Ca-fertilizer, on the secondary metabolism of phenolics in T. grandis nuts is largely unknown, for which transcriptomic and metabolomic analysis was carried out.
View Article and Find Full Text PDFIntegrated transcriptomic and metabolomic analyses elucidate the mechanism by which grafting impacts potassium utilization efficiency in tobacco.
BMC Plant Biol
January 2025
College of Economic and Management, Henan Agricultural University, Zhengzhou, 450002, China.
Background: Potassium plays a crucial role in determining the quality of flue-cured tobacco leaves. Our prior investigations have demonstrated that using potassium-efficient rootstocks through grafting offers a viable solution to the prevalent issue of low potassium levels in Chinese flue-cured tobacco leaves. Nevertheless, the specific molecular mechanisms responsible for the increase in potassium content following grafting in tobacco leaves have yet to be elucidated.
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