Synthesis of halogenated azo-aspirin analogues from natural product derivatives as the potential antibacterial agents.

Chemical modification of medicines from natural product-based molecules has become of interest in recent years. In this study, a series of halogenated azo derivatives were synthesised via coupling reaction, followed by Steglich esterification with aspirin (a natural product derivative) to form azo derivatives . While, halogenated azo-aspirin were synthesised via direct coupling reaction of aspirin and diazonium salt. Bacteriostatic activity was demonstrated against and via turbidimetric kinetic method. Compound showed excellent antibacterial activities against (MIC 75-94 ppm) and (MIC 64-89 ppm) compared to ampicillin (MIC 93 and 124 ppm respectively), followed by and The presence of reactive groups of -OH, N=N, C=O and halogens significantly contribute excellent interaction towards and . Molecular dockings analysis of against MIaC protein showed binding free energy of -7.2 kcal/mol ( and -6.6 kcal/mol (.