Alginate, an anionic heteropolysaccharide extracted from natural brown algae, has useful properties for the food, chemical, medical, and agricultural industries. Degradation of alginate by alginate lyase is a key process to produce unsaturated oligoalginate and unsaturated monosaccharide 4-deoxy-l-erythro-5-hexoseulose uronic acid. Alginate lyases belonging to the polysaccharide lyase family 7 have been found in, and isolated from, organisms thriving in various environments. Furthermore, research on their function and structure has also progressed well. Here, the preparation of native and recombinant PL-7 alginate lyases and the methods for evaluation of enzymatic activity are summarized. Examples of PL-7 alginate lyase applications are also described.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/bs.mie.2018.01.030 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Directed Evolution of an Alginate Lyase from sp. for Seaweed Fertilizer Production from the Brown Seaweed .
J Agric Food Chem
January 2025
College of Food Science and Nutritional Engineering, China Agricultural University, No.17 Qinghua East Road, Haidian District, Beijing 100083, China.
An alginate lyase (FsAly7) from sp. was engineered by directed evolution to improve its optimum temperature and thermostability. The optimum temperature of the positive mutant mFsAly7 (FsAly7-Ser43Pro) was increased by 5 °C, and the thermal inactivation half-lives at 40 and 45 °C were 4.
View Article and Find Full Text PDFA bifunctional endolytic alginate lyase with two different lyase catalytic domains from sp. H204.
Front Microbiol
December 2024
Key Laboratory of Agro-Products Processing Technology of Shandong Province, Key Laboratory of Novel Food Resources Processing Ministry of Agriculture, Institute of Food & Nutrition Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, China.
Alginate lyases can fully degrade alginate into various size-defined unsaturated oligosaccharide products by -elimination. Here, we identified the bifunctional endolytic alginate lyase Aly35 from the marine bacterium sp. Strain H204.
View Article and Find Full Text PDFGenome Analysis of a Polysaccharide-Degrading Bacterium sp. HZ11 and Degradation of Alginate.
Mar Drugs
December 2024
Yantai Key Laboratory of Characteristic Agricultural Bioresource Conservation & Germplasm Innovative Utilization, School of Life Sciences, Yantai University, Yantai 264000, China.
Marine bacteria are crucial sources of alginate lyases, which play an essential role in alginate oligosaccharide (AOS) production. This study reports the biochemical characteristics of a new species of the genus, sp. HZ11.
View Article and Find Full Text PDFMicrobial enzymes as powerful natural anti-biofilm candidates.
Microb Cell Fact
December 2024
Department of Microbiology and Immunology, Faculty of Pharmacy, Delta University for Science and Technology, International Coastal Road, Gamasa, 11152, Egypt.
Bacterial biofilms pose significant challenges, from healthcare-associated infections to biofouling in industrial systems, resulting in significant health impacts and financial losses globally. Classic antimicrobial methods often fail to eradicate sessile microbial communities within biofilms, requiring innovative approaches. This review explores the structure, formation, and role of biofilms, highlighting the critical importance of exopolysaccharides in biofilm stability and resistance mechanisms.
View Article and Find Full Text PDFExpression, Purification, and Characterisation of Recombinant Alginate Lyase ( AL2) for the Bioconversion of Alginate into Alginate Oligosaccharides.
Molecules
November 2024
Enzyme Science Programme (ESP), Department of Biochemistry, Microbiology and Bioinformatics, Rhodes University, Makhanda 6140, South Africa.
Alginate, a polysaccharide found in brown seaweeds, has regularly gained attention for its potential use as a source of bioactive compounds. However, it is structurally complex with a high molecular weight, limiting its application. Alginate oligosaccharides (AOS) are small, soluble fragments, making them more bioavailable.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!