Several modifications of the roll-tube method have made it simpler for routine use in the isolation and growth of anaerobic bacteria. These include use of a check valve for the production of prereduced anaerobically sterilized media; a Salvarsan tube under oxygen-free gas pressure for the dispensing of molten prereduced anaerobically sterilized agar medium; a Kelly infusion bottle with a graduated pipette side arm (also under gas pressure) for quantitative delivery of fluid prereduced anaerobically sterilized media; and screw-capped prescription bottles for the cultivation of anaerobes. Colonies of Bacteroides melaninogenicus were easily identified and counted by this method. Other anaerobic bacteria have also been cultivated successfully.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC275165 | PMC |
| http://dx.doi.org/10.1128/jcm.8.2.123-126.1978 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Detection of Psychrophilic Clostridium spp. in Fecal Samples from Cattle of Different Ages Sampled at the Slaughterhouse Level.
J Food Prot
January 2021
Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 272, 8057, Zurich, Switzerland (ORCID: https://orcid.org/0000-0002-6071-5505 [J.W.]; https://orcid.org/0000-0003-1002-4762 [R.S.]).
Article Synopsis
- The study investigates the presence of Clostridium estertheticum and similar bacteria in the feces of cattle at a slaughterhouse, which can spoil vacuum-packed meat.
- A total of 102 fecal samples were collected, with 39% testing positive for these bacteria, showing varying infection rates depending on the age of the cattle.
- Six strains of Clostridium were isolated and identified, highlighting the significance of cattle feces as a source of spoilage bacteria and emphasizing the need for strict hygiene practices in slaughterhouses to prevent carcass contamination.
Electron transfer kinetics of the mitochondrial outer membrane protein mitoNEET.
Free Radic Biol Med
June 2018
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA. Electronic address:
Increasing evidence suggests that the mitochondrial outer membrane protein mitoNEET is a key regulator of energy metabolism, iron homeostasis, and production of reactive oxygen species in mitochondria. Previously, we reported that mitoNEET is a redox enzyme that catalyzes electron transfer from the reduced flavin mononucleotide (FMNH) to oxygen or ubiquinone via its unique [2Fe-2S] clusters. Here, we explore the reduction and oxidation kinetics of the mitoNEET [2Fe-2S] clusters under anaerobic and aerobic conditions.
View Article and Find Full Text PDFToxin production of Clostridium difficile in sub-MIC of vancomycin and clindamycin alone and in combination with ceftazidime.
Microb Pathog
June 2017
Department of Microbiology and Virology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran. Electronic address:
Toxin production in Clostridium difficile (C. difficile) is a key process for induction of diarrhea. Several factors such as sub-MIC of antibiotics impact on toxin production.
View Article and Find Full Text PDFIsolation and characterization of new strains KOR-3, -4, -5, and -6 from an anaerobic digester using pig slurry.
Asian-Australas J Anim Sci
August 2017
Biogas Research Center, Hankyong National University, Anseong 17579, Korea.
Objective: An experiment was conducted to isolate and identify new methanogens in Korea from an anaerobic digester that uses pig slurry.
Methods: An anaerobic digestate sample was collected from an anaerobic digester using pig slurry. Pre-reduced media were used for the growth and isolation of methanogens.
Investigation of a Candida guilliermondii Pseudo-outbreak Reveals a Novel Source of Laboratory Contamination.
J Clin Microbiol
April 2017
Harvard Medical School, Boston, Massachusetts, USA
was isolated from sterile specimens with increasing frequency over a several-month period despite a paucity of clinical evidence suggesting true infections. However, a health care-associated outbreak was strongly considered due to growth patterns in the microbiology laboratory that were more consistent with true infection than environmental contamination. Therefore, an extensive investigation was performed to identify its cause.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!