This study aimed to evaluate the effect of equine chorionic gonadotrophin (eCG) stimulation prior to ovum pick-up (OPU) on follicular development, number and quality of recovered oocytes, fertilization rate, and early embryo development in vitro. There were four OPU sessions (cross over) conducted on 16 Braford cows to evaluate the effect of various eCG doses. The timing of the wave of ovarian follicular development was synchronized, and three days after, the respective eCG dose was administrated (0, 200, 400, or 800 IU). The OPU was performed on Day 6, and viable oocytes were used for IVM and IVF according to the respective treatment. After IVF treatment, the fertilization and cleavage rates, time of cleavage, and the cell number at 48 h were evaluated. There was no difference in the number of follicles, oocyte quantity, and morphological quality of oocytes among treatments (P > 0.05). The oocyte recovery rate was similar among the eCG-treated groups, but was less than in the control group (P < 0.01). The eCG800 group, however, had a greater recovery rate of follicles >6 mm in diameter (P < 0.01). In addition, the eCG800 group had a greater rate of normal fertilization (P < 0.01) and lesser rate of polyspermy (P < 0.02). The cleavage rate of the eCG800 group was greater than the other treatment groups but similar to that of the control. In conclusion, the use of eCG800 increased the proportion of follicles > 6 mm, with improved rate of normal fertilization and reduced occurrence of polyspermy, without affecting early embryonic development in vitro.
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http://dx.doi.org/10.1016/j.anireprosci.2018.06.006 | DOI Listing |
Theriogenology
January 2025
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 08826, Seoul, Republic of Korea. Electronic address:
Follicular fluid-derived meiosis-activating sterol (FF-MAS), an intermediate in the cholesterol biosynthesis pathway, plays a crucial role in the meiotic resumption of mammalian oocytes. Maintaining a high concentration of FF-MAS in vitro is challenging; therefore, AY9944 A-7, an inhibitor of Δ14-reductase [which converts FF-MAS to testis meiosis-activating sterol (T-MAS)] and Δ7-reductase (which converts T-MAS to cholesterol), has been used to enhance oocyte maturation. This study examined the effects of various concentrations (0, 10, 20, and 40 μM) of AY9944 A-7 on porcine oocyte maturation and subsequent embryo development.
View Article and Find Full Text PDFJDS Commun
January 2025
Department of Animal Sciences, The Ohio State University, Columbus, OH 43210.
The objective of the present study was to evaluate the efficacy of various methods for synchronization of follicular wave emergence (FWE) in pregnant heifers. Pregnant (60 d of gestation) Holstein heifers (n = 86) arranged in cohorts were randomly assigned to be administered 172 µg of gonadorelin acetate (GnRH), 3,300 IU of human chorionic gonadotropin (hCG), follicular ablation of follicles >5 mm (FA), or saline (control). Ultrasonography was performed to determine ovulation and emergence of a new follicular wave.
View Article and Find Full Text PDFBMC Med
January 2025
Department of Nuclear Medicine, West China Hospital, Sichuan University, Guoxue Alley, Address: No.37, Chengdu City, Sichuan, 610041, China.
Background: This study aimed to construct a radiomics-based imaging biomarker for the non-invasive identification of transformed follicular lymphoma (t-FL) using PET/CT images.
Methods: A total of 784 follicular lymphoma (FL), diffuse large B-cell lymphoma, and t-FL patients from 5 independent medical centers were included. The unsupervised EMFusion method was applied to fuse PET and CT images.
Sci Rep
January 2025
Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, No. 4110 Yatai Street, Nanguan District, Changchun, Jilin, 130000, China.
Polycystic ovary syndrome (PCOS) is a complex gynecological endocrinological condition that significantly impacts women's fertility during their reproductive lifespan. The causes of PCOS are multifaceted, and its pathogenesis is not yet clear. This study established a rat model of PCOS and, in conjunction with clinical samples and database data, analysed the role of claudin 11 (CLDN11) in follicular granulosa cells (GCs) in regulating the proliferation of GCs.
View Article and Find Full Text PDFReprod Toxicol
January 2025
Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Ovarian Preantral Follicles (LAMOFOPA), State University of Ceará, Av. Dr. Silas Munguba, 1700, CEP: 60714-903, Fortaleza, CE, Brazil. Electronic address:
This study aimed to investigate, in vitro, the toxicity of WTA on ovarian follicles. Initially, a cytotoxicity assay was conducted using tumor and non-tumor cell lines to determine the ICof the WTA and validate its antitumor activity. Mouse ovaries were cultured in vitro (IVC) for 6 days in the presence of 1% dimethyl sulfoxide (DMSO), doxorubicin at 0.
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