AI Article Synopsis
- - The study assessed how higher temperatures affect bovine oviduct epithelial cells (BOECs), focusing on two proteins: heat shock protein 70 (HSP70) for cell defense and oviduct specific glycoprotein 1 (OVGP1) for embryo support.
- - BOECs were tested at normal (38.5°C) and elevated temperatures (41°C) for 168 hours, revealing that while BOECs remained viable, embryo development suffered negatively at higher temperatures.
- - Increased HSP70 and decreased OVGP1 expression were observed at elevated temperatures, but co-culturing with embryos helped maintain some OVGP1 levels, highlighting the complex relationship between embryo development and oviduct conditions under
Article Abstract
The aim of this study was to evaluate the influence of elevated temperature on bovine oviduct epithelial cells (BOECs), based on the expression and localization of both heat shock protein 70 (HSP70), responsible for the cellular defence mechanism, and oviduct specific glycoprotein 1 (OVGP1) which is the most important embryotrophic protein. BOECs were cultured alone and co-cultured with cattle embryos at control (38.5°C) and elevated temperature (41°C) for 168 h. The elevated temperature had no effect on the viability of BOECs but exerted a negative effect on embryo development. The elevated temperature increased the expression of HSP70 and decreased the expression of OVGP1 at both mRNA and protein levels in BOECs cultured alone and those co-cultured with embryos. However, the presence of embryos limited the decrease in OVGP1 expression in BOECs at elevated temperature but did not alter the expression of HSP70. These results demonstrate for the first time the influence of elevated temperature on BOECs, consequently providing insights into the interactions between the embryo and the oviduct at elevated temperature.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6003681 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0198843 | PLOS |
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