Objective: To develop an indirect competitive chemiluminescent enzyme immunoassay( CLEIA) for the detection of bisphenol A in milk samples.
Methods: The CLEIA conditions including antigen coated concentration, concentration of methanol, concentration of enzyme labeled anti-antibody, p H and ionic strength were optimized to build competitive inhibition curve, determine the linear range and detection limit, and study the recovery of spiked milk samples.
Results: In the standard curve of the optimized CLEIA, the half maximal inhibitory concentration( IC_(50)) was 3. 95 ng/m L and the limit of detection( LOD) was 0. 66 ng/m L. The CLEIA showed good recoveries with spiked milk ranging from 95. 0%-112. 9% with the relative standard deviation of0. 93%-3. 96%.
Conclusion: The proposed method has a high sensitivity and good specificity and is suitable for the determination of bisphenol A in milk samples.
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