Background: An outbreak of gastroenteritis (GE) occurred in community-dwelling adults in the Central Region of urban Singapore, in May 2016.
Objectives: To investigate the cause of the outbreak.
Study Design: We conducted a case-cohort study on GE patients linked to the outbreak who presented to the emergency department of a tertiary-care hospital near the outbreak area, from 18 May to 11 June 2016. Stools were tested for gastrointestinal pathogens including rotavirus antigen and positive rotavirus samples were subject to genotyping.
Results: A total of 57 adult GE patients, with a median age of 40 (range 18 to 84) years, were included. Predominant symptoms were diarrhoea (98.2%), vomiting (64.9%), and abdominal discomfort (38.6%). Age 65 years and above (Adjusted OR 21.78, 95% CI 1.49-318.84; P = 0.02) was the only predictor of admission, after adjusting for comorbidities and clinical severity. Molecular microbiological investigations confirmed that the outbreak was caused by a novel human-bovine reassortant strain of rotavirus G8P[8] with DS-1-like backbone. Exposure to the market in the outbreak area was strongly associated with rotavirus infection (OR 46.14; 95% CI 43.04-49.25, P < 0.01). No particular food item could be identified as the outbreak cause.
Conclusions: This is the first report of an outbreak of rotavirus G8P[8] in adults in an urban community that is not waterborne. Transmission was likely through fomites in the market and its surrounding areas, via consumption or contact with contaminated food items purchased from the market, and from person-to-person. The potential for novel G8P[8] strains to cause outbreaks cannot be overemphasized.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jcv.2018.06.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Prevalence and genetic characterization of viral gastroenteritis in hospitalized children aged <5 years in Yunnan Province, China, 2020-2022.
Front Pediatr
January 2025
Yunnan Provincial Key Laboratory of Public Health and Biosafety & Institute for AIDS/STD Control and Prevention, Yunnan Center for Disease Control and Prevention, Kunming, Yunnan, China.
Article Synopsis
- The study focused on the prevalence of various viruses causing acute gastroenteritis (AGE) in children under five years old in Yunnan, China.
- Over 610 stool samples were analyzed, revealing that rotavirus (RVA) was present in nearly 29% of the cases, with G8P[8] emerging as the dominant genotype in 2022.
- Norovirus was detected in about 12% of the samples, with GII.4 being the most common strain, while other viruses like sapovirus and human adenovirus had lower positivity rates.
- The results indicated higher AGE incidence in male children, particularly among those aged 12-23 months, with infection rates peaking in the early months of the year, highlighting
Genomic analysis of DS-1-like human rotavirus A strains uncovers genetic relatedness of NSP4 gene with animal strains in Manhiça District, Southern Mozambique.
Sci Rep
December 2024
Centro de Investigação em Saúde de Manhiça (CISM), Maputo, Mozambique.
Post rotavirus vaccine introduction in Mozambique (September 2015), we documented a decline in rotavirus-associated diarrhoea and genotypes changes in our diarrhoeal surveillance spanning 2008-2021. This study aimed to perform whole-genome sequencing of rotavirus strains from 2009 to 2012 (pre-vaccine) and 2017-2018 (post-vaccine). Rotavirus strains previously detected by conventional PCR as G2P[4], G2P[6], G3P[4], G8P[4], G8P[6], and G9P[6] from children with moderate-to-severe and less-severe diarrhoea and without diarrhoea (healthy community controls) were sequenced using Illumina MiSeq platform and analysed using bioinformatics tools.
View Article and Find Full Text PDFPrevalence, genotype diversity, and zoonotic potential of bovine rotavirus A in Amhara National Regional State, Ethiopia: A multicenter cross-sectional study.
Virus Res
December 2024
Center for Food Animal Health, Department of Animal Sciences, College of Food Agricultural and Environmental Sciences, The Ohio State University, Wooster, OH 44691, USA; Department of Veterinary Preventive Medicine, The College of Veterinary Medicine, The Ohio State University, Wooster, OH 44691, USA. Electronic address:
Article Synopsis
- Rotavirus A (RVA) is a leading cause of gastroenteritis in calves and has the potential to spread to humans, making it a public health concern.
- A study in Amhara, Ethiopia, involved 266 calves to assess the prevalence and genetic variety of RVA, finding a 15.4% infection rate with various G and P genotypes.
- The research highlights the need for ongoing monitoring of RVA in calves due to its significant presence and possible zoonotic transmission risks.
Identification of Rotavirus Genotypes in Children under Five Years in the United Arab Emirates Using Nanopore Sequencing Technology.
J Med Virol
November 2024
Department of Pediatrics, College of Medicine and Health Sciences, UAE University, Al-Ain, UAE.
Article Synopsis
- Despite universal vaccination, Group A rotaviruses (RVA) continue to cause significant childhood diarrhea in the UAE, highlighting the need for genetic monitoring to track prevalent strains and potential vaccine escape mutants.
- The study compared real-time polymerase chain reaction (RT-PCR) and nanopore sequencing for genotyping 33 RVA strains from children, finding that RT-PCR could miss some new strains, whereas nanopore sequencing identified more diverse genotypes.
- The results showed that nanopore sequencing was more effective in revealing emerging strains, with G3P[8] being the most common, thus suggesting its potential role in improving RVA surveillance and guiding preventive strategies.
Article Synopsis
- Viral enteric infections, caused by norovirus, rotavirus A, and human adenovirus, significantly impact the gastrointestinal system, highlighting the need for rapid diagnostic tools like the immunochromatographic (IC) test.
- The IP-Triple I IC test kit was evaluated against known positive stool specimens and showed 100% specificity for detecting NoV, RVA, and HAdV, with varying sensitivity levels of 86.7% for RVA, 70.6% for NoV, and 76.2% for HAdV.
- The findings confirm that the IP-Triple I IC test kit is effective for quick detection of multiple viral genotypes in stool samples, proving valuable for timely diagnosis during outbreaks.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!