Retail seafood in Berlin, Germany, was investigated to detect the prevalence and quantitative load of Enterobacteriaceae that produce extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase (AmpC). A total of 160 raw seafood samples were screened for the presence of these bacteria using MacConkey agar supplemented with 1 mg/L cefotaxime after nonselective enrichment. Isolated species were subsequently identified using matrix-assisted laser desorption-ionization time-of-flight analysis. ESBL and AmpC production was tested by the disk diffusion method, and ESBL and AmpC genes were characterized using real-time and conventional PCR assays with DNA sequencing. Spread plating was used for quantification of ESBL- and AmpC-producing Enterobacteriaceae. Overall, these bacteria were detected in 21.3% of seafood samples (34 of 160 samples) with prevalences of 22.5 and 20% for shrimp and bivalves, respectively. Of the positive samples, 91.2% contained an ESBL- or AmpC-producing Enterobacteriaceae load of <100 CFU/g (lower detection limit), and 8.8% contained 100 to 1,000 CFU/g. Among the 45 Enterobacteriaceae isolates, Klebsiella pneumoniae (13 isolates) and Escherichia coli (12 isolates) were the predominant species. ESBL and AmpC genes were detected in 33 isolates, with the majority of isolates harboring bla (27.3%), bla (21.2%), or bla (21.2%). Our study highlights the hazard associated with seafood containing ESBL- and AmpC-producing Enterobacteriaceae in Germany. Even though the contamination levels were low, the high prevalence of ESBL- and AmpC-producing Enterobacteriaceae in seafood might be of concern to public health because of the potential transmission of these bacteria from seafood to humans through the food chain.
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http://dx.doi.org/10.4315/0362-028X.JFP-18-029 | DOI Listing |
Cureus
December 2024
Clinical Microbiology, Prathima Institute of Medical Sciences, Karimnagar, IND.
Introduction Intestinal carriage of multidrug-resistant organisms (MDROs) in healthy populations could amplify resistant bacteria, which may increase the risk of infections by these bacteria in the community and in the hospital. This study investigated the prevalence of colonization of multidrug-resistant (MDR) bacteria in the intestines of healthy individuals in South India. Methods A prospective study was conducted for six months at a tertiary care teaching hospital.
View Article and Find Full Text PDFJ Infect Chemother
December 2024
Department of Pediatrics, Dokkyo Medical University, Tochigi, Japan.
The incidence of urinary tract infection (UTI) caused by extensive beta-lactamase-producing Escherichia coli (ESBL-EC) is increasing, including in children. However, the available oral antibiotic treatment options for ESBL-EC are limited. Herein, we report the cases of two children diagnosed with UTI caused by ESBL-EC (ESBL-UTI) who were switched from empirical intravenous antibiotics in UTI to amoxicillin-clavulanic acid (AMPC/CVA) (14:1) after the causative organism was found to be ESBL-EC.
View Article and Find Full Text PDFRinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi
December 2024
Department of Clinical Laboratory, Chiba Municipal Hospital.
Bacteremia can easily become severe, and it is necessary to start appropriate treatment quickly before it progresses to sepsis. In this study, we investigated a method using antibiotic discs to quickly report antibiotic resistance information for bacteremia caused by gramnegative bacilli, such as , which require careful selection of appropriate antibiotics due to their tendency to cause severe illness. In cases of bacteremia caused by ESBL and AmpC-producing bacteria, reports of suspected antibiotic resistance mechanisms were made within a few hours after disk placement, allowing for the rapid modification of antimicrobial therapy, thereby contributing to the treatment.
View Article and Find Full Text PDFJ Dairy Sci
December 2024
Royal GD, PO Box 9, 7400 AA Deventer, the Netherlands.
The duration of excretion of dry cow antimicrobials is hard to predict and therefore the level of antimicrobial residues in colostrum can differ considerably between cows. The feeding of colostrum with antimicrobial residues to newborn calves may lead to antimicrobial resistance in calves. The aim of this study was to quantify the prevalence and residue level of dry cow antimicrobials in colostrum from dairy cows.
View Article and Find Full Text PDFFoods
December 2024
Centre for Applied Food Safety and Biotechnology, Department of Life Sciences, Central University of Technology, 1 Park Road, Bloemfontein 9300, South Africa.
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