The objective of this study was to determine whether endophytic fungi, isolated from produce nigranoic acid and its highly oxygenated derivatives. From the 426 endophytic fungi screened, SWUKD3.1610 was detected to have a component with the same TLC value and HPLC retention time as authentic nigranoic acid. This component was further confirmed as nigranoic acid by investigating the chemical composition of the fungal extracts. Besides (), one new triterpenoid, 7- schinalactone C (), and two known minor compounds were isolated and characterized by HRESIMS, 1D and 2D NMR spectroscopic methods. Our study indicates that endophytic fungus may play an important role in increasing the quality of the crude drugs from Chinese medicinal plant . This study is the first to isolate, characterize, and identify schitriterpenes-producing .
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http://dx.doi.org/10.1080/14786419.2018.1486311 | DOI Listing |
Zhongguo Zhong Yao Za Zhi
March 2024
Traditional Chinese Medicine and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China.
The dichloromethane fraction of Kadsura heteroclita roots was separated and purified by chromatographic techniques(e.g., silica gel, Sephadex LH-20, ODS, MCI column chromatography) and semi-preparative HPLC.
View Article and Find Full Text PDFBioorg Chem
April 2021
Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, Yunnan Research & Development Center for Natural Products, School of Chemical Science and Technology, Yunnan University, Kunming 650091, PR China. Electronic address:
As a successful anti-tumor drug target, the family of histone deacetylases (HDACs) is also a critical player in immune response, making the research of anti-inflammatory HDAC inhibitors an attractive new focus. In this report, triterpenoids nigranoic acid (NA) and manwuweizic acid (MA) were identified as HDAC inhibitors through docking-based virtual screening and enzymatic activity assay. A series of derivatives of NA and MA were synthesized and assessed for their biological effects.
View Article and Find Full Text PDFPhytochemistry
June 2020
Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, School of Life Sciences, Southwest University, Chongqing, 400715, People's Republic of China. Electronic address:
The fermentation of Kadsura angustifolia with an endophytic fungus, Penicillium ochrochloron SWUKD4.1850 yielded five additional undescribed oxygenated terpenoids, kadanguslactones A-E, together with ten known compounds. Their structures were established by the extensive 1D, 2D-NMR, HR-ESI-MS, CD and X-ray crystallography data analysis.
View Article and Find Full Text PDFFront Microbiol
November 2018
Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Life Sciences, Southwest University, Chongqing, China.
Fungal endophytes live widely inside plant tissues and some have been revealed to provide benefits to their host and ecological environment. Considering the fact that endophytes are engaged in remarkably stable long-term interactions with the host for their whole life cycle, it's conceivable that both partners have substantial influence on each other's metabolic processes. Here, we investigated the fermented products of an endophytic fungus SWUKD3.
View Article and Find Full Text PDFPhytochemistry
February 2019
Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Life Science, Southwest University, Chongqing 400715, China. Electronic address:
Highly oxygenated schitriterpenoids are interesting for study of their structures, bioactivities and synthesis. From Kadsura angustifolia fermented by an associated symbiotic endophytic fungus, Penicillium sp. SWUKD4.
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