AI Article Synopsis
Article Abstract
The molecular features of hepatocellular carcinoma arising from non-alcoholic fatty liver disease (NAFLD-HCC) are not well known. In this study, we investigated the mechanism by which NAFLD-HCC survives in a fat-rich environment. We found that caveolin (CAV)-1 was overexpressed in clinical specimens from NAFLD-HCC patients. HepG2, HLE, and HuH-7 HCC cell lines showed decreased proliferation in the presence of the saturated fatty acids palmitic acid and stearic acid, although only HLE cells expressed high levels of CAV-1. HLE cells treated with oleic acid (OA) showed robust proliferation, whereas CAV-null HepG2 cells showed reduced proliferation and increased apoptosis. CAV-1 knockdown in HLE cells attenuated the OA-induced increase in proliferation and enhanced apoptosis. Liquid chromatography-tandem mass spectrometry analysis revealed that the levels of OA-containing ceramide, a pro-apoptotic factor, were higher in HepG2 and CAV-1-deficient HLE cells than in HLE cells, suggesting that CAV-1 inhibits apoptosis by decreasing the level of OA-containing ceramide. These results indicate that CAV-1 is important for NAFLD-HCC survival in fatty acid-rich environments and is a potential therapeutic target.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6113505 | PMC |
| http://dx.doi.org/10.1111/cas.13659 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Effects of Dexamethasone on Human Lens Epithelial Cells and the Analysis of Related Pathways with Transcriptome Sequencing.
Front Biosci (Landmark Ed)
November 2024
Department of Ophthalmology, University-Town Hospital of Chongqing Medical University, 401331 Chongqing, China.
Background: The goal of this study was to investigate the effects of dexamethasone on human lens epithelial cells (HLECs) and the potential mechanisms.
Methods: HLECs (HLE-B3) were cultured to assess the effects of dexamethasone on cell size at different concentrations. Immunofluorescence staining was used to detect specific protein expression in HLE-B3 cells.
Effects of MMP2 and its inhibitor TIMP2 on DNA damage, apoptosis and senescence of human lens epithelial cells induced by oxidative stress.
J Bioenerg Biomembr
December 2024
Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, Tianjin, 300384, China.
Oxidative stress-induced lens epithelial cells (LECs) death plays a pivotal role in pathogenesis of age-related cataract (ARC), causing significant visual impairment. Apoptosis of porcine granulosa cells mediated by MMP2 is linked to DNA damage. The current study aimed to investigate the potential mechanism of MMP2 in DNA damage, apoptosis and senescence of lens epithelial cells caused by oxidative stress.
View Article and Find Full Text PDFFangchinoline suppresses hepatocellular carcinoma by regulating ROS accumulation via the TRIM7/Nrf2 signaling pathway.
Phytomedicine
December 2024
School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, 330031, China. Electronic address:
Article Synopsis
- Dysregulation of redox homeostasis and oxidative stress are linked to the development of hepatocellular carcinoma (HCC), with Fangchinoline (FAN) being a potential anti-cancer drug not yet fully studied for its effects on oxidative stress.
- The research aims to investigate how FAN can prevent HCC by regulating oxidative stress and to uncover the molecular mechanisms involved, using both animal models and cell lines.
- Findings indicate that FAN inhibits cell proliferation and tumor formation in HCC by promoting ROS accumulation, activating the Nrf2 pathway, and engaging TRIM7, which helps stabilize Nrf2 through a feedback loop.
An Innovative Approach to Optimize First-In-Human Minimal Anticipated Biological Effect Level Based Starting Dose in Oncology Trials for Bispecific T-Cell Engagers: Experience from A Solid Tumor Bispecific T-Cell Engager.
Clin Pharmacol Ther
January 2025
Clinical Pharmacology Modeling & Simulation, Amgen Inc., South San Francisco, California, USA.
Article Synopsis
- Bispecific T-cell engagers (Bi-TCEs) are innovative cancer treatments that have shown promise for both blood cancers and solid tumors, but starting dose selection for early human trials is complex due to risks of toxicity and ineffectiveness.
- The traditional method for determining doses often leads to long escalation periods, while a new modified approach uses better-targeted biological data and dosages that match the tumor environment, minimizing risks.
- This new method was successfully applied in a trial for a Bi-TCE targeting gastric cancer, allowing for a much higher initial dose that was safe and well-tolerated, which could expedite future clinical developments for Bi-TCEs in various cancer types.
Protective effect of ghrelin in oxidative stress-induced age-related macular degeneration in vitro and in vivo.
Mol Med
September 2024
Department of Oral Pathology, School of Stomatology, Hainan Medical University, Haikou, 571199, P. R. China.
Article Synopsis
- Oxidative damage to retinal pigment epithelial (RPE) cells is a key factor in age-related macular degeneration (AMD), a condition affecting vision as people age.
- Previous research indicated that ghrelin, a hormone, has antioxidant properties in human lens epithelium (HLE) cells, but its effects on retinal diseases had been underexplored.
- This study found that ghrelin protects ARPE-19 cells from oxidative stress and cell death induced by hydrogen peroxide, and it also preserved retinal function in a mouse model of AMD when administered.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!