AI Article Synopsis
- - Rabbits are important for testing how chemicals can cause developmental abnormalities, known as teratogenicity, which is vital for understanding risks to humans.
- - When teratogenic effects are found, it's crucial to study the underlying mechanisms that could affect human health.
- - The chapter discusses using a method called whole-mount in situ hybridization (WISH) with a specialized probe to visualize gene and protein expression in rabbit embryos for detailed analysis.
Article Abstract
Rabbits are valuable experimental animals used to evaluate the teratogenicity of chemical entities. If teratogenicity is recognized, it is very important to investigate the pathogenesis to assess the potential risk in humans. For this investigation, whole-mount in situ hybridization (WISH) is highly useful for visualizing spatial and temporal changes in the expression patterns of the targeted genes and or proteins. In this chapter, the WISH method in rabbit embryos, using a digoxigenin-labeled RNA probe, and the morphometric analysis of the expression pattern is described.
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| http://dx.doi.org/10.1007/978-1-4939-7883-0_34 | DOI Listing |
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