Background: To study structural changes in naïve and surgically treated corneas of aniridia patients with advanced aniridia-related keratopathy (ARK).
Methods And Findings: Two naïve corneal buttons from patients with advanced ARK submitted to penetrating keratoplasty for the first time, one corneal button from an ARK patient that had undergone a keratolimbal allograft (KLAL), two corneal buttons from ARK patients who had previously undergone centered or decentered transplantation and were now retransplanted and two adult healthy donor control corneas were processed for immunohistochemistry. Antibodies against extracellular matrix components in the stroma and in the epithelial basement membrane (collagen I and IV, collagen receptor α11 integrin and laminin α3 chain), markers of fibrosis, wound healing and vascularization (fibronectin, tenascin-C, vimentin, α-SMA and caveolin-1), cell division (Ki-67) and macrophages (CD68) were used. Naïve ARK, KLAL ARK corneas and transplanted corneal buttons presented similar histopathological changes with irregular epithelium and disruption or absence of epithelial basal membrane. There was a loss of the orderly pattern of collagen lamellae and absence of collagen I in all ARK corneas. Vascularization was revealed by the presence of caveolin-1 and collagen IV in the pannus of all ARK aniridia corneas. The changes observed in decentered and centered transplants were analogous.
Conclusions: Given the similar pathological features of all cases, conditions inherent to the host seem to play an important role on the pathophysiology of the ARK in the long run.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5995400 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0198822 | PLOS |
Publication Analysis
Top Keywords
Similar Publications
Femtosecond laser-assisted large-diameter lamellar corneal-limbal keratoplasty in ocular chemical burns.
Am J Ophthalmol Case Rep
March 2025
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou, China.
Purpose: This study highlights the feasibility of femtosecond laser-assisted large-diameter lamellar corneal-limbal keratoplasty and its efficacy in the treatment of ocular surface failure caused by bilateral ocular chemical injury.
Observations: The series included 3 patients with ocular surface failure caused by bilateral ocular chemical burns. After dissection of the host cornea, a femtosecond laser-assisted large-diameter lamellar corneoscleral button, with varying thickness of 250-400 μm, was sutured to the recipient bed.
Comparison of Intraocular Pressure Measurements Using Three Different Methods (Goldmann Applanation Tonometry (GAT), Corvis ST, and iCare) Following Penetrating Keratoplasty.
J Clin Med
November 2024
2nd Department of Ophthalmology, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Intraocular pressure (IOP) readings using three different methods (Goldmann applanation tonometry (GAT), Corvis ST, and iCare) were compared in patients who underwent penetrating keratoplasty (PK). : An observational cross-sectional study with prospective recruitment of patients was conducted. IOP measurements were acquired using GAT, iCare, and Corvis (including both uncorrected IOP (CVS-IOP) and biomechanical IOP (bIOP)), and the agreement among methods was analyzed using Bland-Altman plots.
View Article and Find Full Text PDFInhibition of matrix metalloproteases by a chemical cross-linker to halt the corneal degradation in keratoconus.
Exp Eye Res
December 2024
Aravind Medical Research Foundation, Madurai, Tamil Nadu, India. Electronic address:
The need for better and simpler alternative crosslinking strategies to treat keratoconus (KC) is becoming essential as there is only a single approved way to treat it. Recently, conventional UV-A Riboflavin crosslinking is proven to have some disadvantages such as causing damage to the corneal endothelium and inducing keratocyte apoptosis. A chemical cross-linker (CXL) using carbodiimide chemistry and an octanedioic acid spacer is found effective in stiffening the cornea and has the potential to be developed as an alternative therapy to halt KC progression.
View Article and Find Full Text PDFTransmission Rates of UV-A and Green Light in an ex vivo Corneal Cross-linking Model for Infectious Keratitis.
J Cataract Refract Surg
December 2024
ELZA Institute, Dietikon, Switzerland.
Purpose: To investigate the light transmission (LT) of UV-A and green light through infected corneas saturated with riboflavin or rose bengal in an ex vivo porcine model for infectious keratitis.
Setting: University of Zurich and EMPA.
Design: Laboratory study.
Five Year Review of All Examined Corneal Tissue in a Tertiary Eye Care Center: Demographics and Surgical Indications.
J Epidemiol Glob Health
December 2024
Ophthalmology Department, King Saud University, Riyadh, Saudi Arabia.
Background: Corneal pathologies are among the most common reversible sight-threatening diseases globally. As such, corneal tissue transplantation (keratoplasty) techniques are evolving over time depending on the prevalence of different pathologies in each geographical area. We aim to provide a baseline information on the common keratoplasty procedures performed in our area in relation to prevalent corneal pathologies and to analyze common corneal surgical practice trends in our area.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!