perfectBASH: Band-selective homonuclear decoupling in peptides and peptidomimetics.

Band selective techniques offer the highest sensitivity of all pure shift approaches and thus are the best choice for decoupling well-separated H-frequency regions, such as the amide- or the α-proton region of α-peptides. They are inept to fully decouple the amide- and the α-proton region simultaneously, though. Herein, we present a new homonuclear decoupling technique, which extends the capabilities of band selective decoupling using the perfect echo principle. This modification allows a complete backbone decoupling (amide- and α-protons) in peptides and opens band selective homonuclear decoupling to substances with two mutually coupled protons in the spectral range of interest.