AI Article Synopsis
- Malic acid carbon dots show superior photoblinking properties compared to traditional dyes and are highly biocompatible, making them ideal for use in advanced microscopy techniques in both fixed and live cells.
- They do not exhibit “excitation wavelength-dependent” emission, which prompted the development of a quick and efficient method to separate them by particle size using C reversed-phase silica gel column chromatography.
- This separation technique helps in understanding how different particle sizes affect their optical properties, such as band gap energies and photoluminescence behaviors.
Article Abstract
As-synthesized malic acid carbon dots are found to possess photoblinking properties that are outstanding and superior compared to those of conventional dyes. Considering their excellent biocompatibility, malic acid carbon dots are suitable for super-resolution fluorescence localization microscopy under a variety of conditions, as we demonstrate in fixed and live trout gill epithelial cells. In addition, during imaging experiments, the so-called "excitation wavelength-dependent" emission was not observed for individual as-made malic acid carbon dots, which motivated us to develop a time-saving and high-throughput separation technique to isolate malic acid carbon dots into fractions of different particle size distributions using C reversed-phase silica gel column chromatography. This post-treatment allowed us to determine how particle size distribution influences the optical properties of malic acid carbon dot fractions, that is, optical band gap energies and photoluminescence behaviors.
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| http://dx.doi.org/10.1021/acsnano.8b01619 | DOI Listing |
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