AI Article Synopsis
- Scientists explored the changes in mitochondrial DNA (mtDNA) levels during early embryogenesis in two plant species, *Torenia fournieri* and *Arabidopsis thaliana*, using advanced techniques like single-cell quantitative PCR and laser confocal microscopy.
- Unlike in mammalian embryos, where mtDNA levels remain constant, the study found that mtDNA undergoes significant doubling during the zygotic phase in *T. fournieri* and during the two-cell stage in *A. thaliana*.
- These findings suggest that the behavior of mtDNA during early development is different in plants compared to mammals, shedding light on the unique dynamics of plant embryogenesis.
Article Abstract
Changes in the amount of mitochondrial DNA (mtDNA) have never been investigated in plant zygotes or early plant embryos due to the difficulty in isolating these cells, although such changes have been investigated in mammalian embryos. Using the single-cell quantitative real-time polymerase chain reaction (PCR) and laser confocal microscopy, we surveyed the changes in mtDNA levels during early embryogenesis in Torenia fournieri and Arabidopsis thaliana. In contrast with the amount of mtDNA in early mammalian embryos, which does not change, we found that mtDNA doubling occurred during zygotic development in T. fournieri and during two-cell proembryo development in A. thaliana. These findings reveal that mtDNA doubling occurs during early embryogenesis in T. fournieri and A. thaliana, indicating that the dynamics of mtDNA in early plant embryos differs from that in early mammalian embryos.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/tpj.13987 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!