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Differential effects of chronic unpredictable stress on behavioral and molecular (cortisol and microglia-related neurotranscriptomic) responses in adult leopard (leo) zebrafish.
Fish Physiol Biochem
January 2025
Neurobiology Program, Sirius University of Science and Technology, Sochi, Russia.
Stress plays a key role in mental, neurological, endocrine, and immune disorders. The zebrafish (Danio rerio) is rapidly gaining popularity as s model organism in stress physiology and neuroscience research. Although the leopard (leo) fish are a common outbred zebrafish strain, their behavioral phenotypes and stress responses remain poorly characterized.
View Article and Find Full Text PDFTransmission of unfolded protein response-a regulator of disease progression, severity, and spread in virus infections.
mBio
January 2025
Department of Infectious Diseases, Molecular Virology, Center for Integrative Infectious Disease Research, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, Germany.
The unfolded protein response (UPR) is a cell-autonomous stress response aimed at restoring homeostasis due to the accumulation of misfolded proteins in the endoplasmic reticulum (ER). Viruses often hijack the host cell machinery, leading to an accumulation of misfolded proteins in the ER. The cell-autonomous UPR is the immediate response of an infected cell to this stress, aiming to restore normal function by halting protein translation, degrading misfolded proteins, and activating signaling pathways that increase the production of molecular chaperones.
View Article and Find Full Text PDFExogenously Applied Triacontanol Mitigates Cadmium Toxicity in L. by Optimizing Growth, Nutritional Orchestration, and Metal Accumulation.
Toxics
December 2024
Department of Industrial Engineering, University of Applied Sciences Technikum Wien, 17 Hoechstaedtplatz 6, 1200 Vienna, Austria.
Cadmium (Cd) is one of the foremost phytotoxic elements. Its proportion in agricultural soil is increasing critically due to anthropogenic activities. Cd stress is a major crop production threat affecting food security globally.
View Article and Find Full Text PDFNanobody-assisted nanoluciferase fragment complementation for in situ measurement and visualization of endogenous protein-protein interaction.
Biosens Bioelectron
December 2024
Shenzhen Bay Laboratory, Shenzhen, 518132, China. Electronic address:
Here, we developed nanobody-assisted nanoluciferase fragment complementation for in situ measurement and visualization of endogenous protein-protein interaction (NanaPPI). When an interaction occurs, primary antibodies for two proteins bring the proximity of secondary nanobody-fused small/large fragment to reassemble into an intact NanoLuc variant, thus transforming interaction events to luminescent signals in situ with high sensitivity. Compared to proximity ligation assay, NanaPPI has a similar signal-to-background ratio, but it is more convenient with faster procedures, easier readout and lower cost.
View Article and Find Full Text PDFA Crop Water Stress Index for Hazelnuts Using Low-Cost Infrared Thermometers.
Sensors (Basel)
December 2024
North Willamette Research and Extension Center, Oregon State University, Aurora, OR 97002, USA.
Incorporating data-driven technologies into agriculture presents a promising approach to optimizing crop production, especially in regions dependent on irrigation, where escalating heat waves and droughts driven by climate change pose increasing challenges. Recent advancements in sensor technology have introduced diverse methods for assessing irrigation needs, including meteorological sensors for calculating reference evapotranspiration, belowground sensors for measuring plant available water, and plant sensors for direct water status measurements. Among these, infrared thermometry stands out as a non-destructive remote sensing method for monitoring transpiration, with significant potential for integration into drone- or satellite-based models.
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