Molecular Assembly of a Durable HRP-AuNPs/PEDOT:BSA/Pt Biosensor with Detailed Characterizations.

Sensors (Basel)

Department of Chemical and Materials Engineering, Tunghai University, Taichung 407, Taiwan.

Published: June 2018

In this study, we provided the detailed characterizations of our recent HRP-AuNPs/PEDOT:BSA/Pt biosensor, constructed through a simple fabrication procedure with improved stability and good sensitivity. Raman and Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy not only confirmed the synthesis of conductive PEDOT where BSA was the template for the polymerization, but also provided further insights into the stable immobilization of AuNP on the PEDOT:BSA film. Scanning electron microscopy revealed that the attachment of AuNPs were stable under a high salt environment. The current technology demonstrates a feasible procedure to form a functional AuNPs/PEDOT:BSA film that has potential applications in the fabrication of various biosensors and electric devices.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6021847PMC
http://dx.doi.org/10.3390/s18061823DOI Listing

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In this study, we provided the detailed characterizations of our recent HRP-AuNPs/PEDOT:BSA/Pt biosensor, constructed through a simple fabrication procedure with improved stability and good sensitivity. Raman and Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy not only confirmed the synthesis of conductive PEDOT where BSA was the template for the polymerization, but also provided further insights into the stable immobilization of AuNP on the PEDOT:BSA film. Scanning electron microscopy revealed that the attachment of AuNPs were stable under a high salt environment.

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A Novel Conductive Poly(3,4-ethylenedioxythiophene)-BSA Film for the Construction of a Durable HRP Biosensor Modified with NanoAu Particles.

Sensors (Basel)

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Department of Chemical and Materials Engineering, Tunghai University, No.1727, Sec.4, Taiwan Boulevard, Xitun District, Taichung 40704, Taiwan.

In this study, we have investigated the contribution of bovine serum albumin (BSA) to the durability of the electrochemically synthesized poly(3,4-ethylenedioxythiophene) (PEDOT) film on a platinum (Pt) electrode. The electrode was capable to effectively adsorb the nano Au particles (AuNPs) to form a uniform layout, which was then able to immobilize the horseradish peroxidase (HRP) to construct a functional HRP/AuNPs/PEDOT(BSA)/Pt biosensor. Cyclic voltammetry was employed to evaluate the performance of the biosensor through the measurement of hydrogen peroxide.

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