Low temperature is a major limiting factor for plant growth and development. Dehydrin proteins are generally induced in response to low-temperature stress. In previous research, a full-length dehydrin gene, PicW2, was isolated from Picea wilsonii and its expression was associated with hardiness to cold. In order to gain insight into the mechanism of low-temperature tolerance by studying its three-dimensional crystal structure, prokaryotically expressed PicW2 dehydrin protein was purified using chitosan-affinity chromatography and gel filtration, and crystallized using the vapour-diffusion method. The crystal grew in a condition consisting of 0.1 M HEPES pH 8.0, 25%(w/v) PEG 3350 using 4 mg ml protein solution at 289 K. X-ray diffraction data were collected from a crystal at 100 K to 2.82 Å resolution. The crystal belonged to space group C121, with unit-cell parameters a = 121.55, b = 33.26, c = 73.39 Å, α = γ = 90.00, β = 109.01°. The asymmetric unit contained one molecule of the protein, with a corresponding Matthews coefficient of 2.87 Å Da and a solvent content of 57.20%. Owing to a lack of structures of homologous dehydrin proteins, molecular-replacement trials failed. Data collection for selenium derivatization of PicW2 and crystal structure determination is currently in progress.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987745 | PMC |
http://dx.doi.org/10.1107/S2053230X18007537 | DOI Listing |
Nature Reserve has complex habitats and rich species diversity but lacks systematic ecological surveys. We focused on Orchid in Nature Reserve, investigated and analyzed Orchid diversity and changes in community structure according to the characteristics of the alpine valleys in the study area, in terms of altitude gradient and habitat type, using the sample line method and the quadrat method. The results showed that 11 genera and 13 species of Orchidaceae were found in the survey, among which the species richness of was the highest, was the second highest, and the species richness of was the lowest, and the species with relative plurality ≥ 10% were (51.
View Article and Find Full Text PDFEcol Evol
August 2024
State Key Laboratory of Tree Genetics and Breeding Chinese Academy of Forestry Beijing China.
To study the interspecific differentiation characteristics of species originating from recent radiation, the genotyping-by-sequencing (GBS) technique was used to explore the kinship, population structure, gene flow, genetic variability, genotype-environment association and selective sweeps of complex with similar phenotypes from a genome-wide perspective. The following results were obtained: 14 populations of complex could be divided into 5 clades; and diverged earlier and were more distantly related to the remaining 6 spruce species. Various geological events have promoted the species differentiation of complex.
View Article and Find Full Text PDFInt J Mol Sci
February 2024
State Key Laboratory of Efficient Production of Forest Resources, College of Forestry, Beijing Forestry University, Beijing 100083, China.
The NAC family of transcription factors (TFs) regulate plant development and abiotic stress. However, the specific function and response mechanism of NAC TFs that increase drought resistance in remain largely unknown. In this study, we functionally characterized a member of the PwNAC family known as .
View Article and Find Full Text PDFHeredity (Edinb)
August 2023
Key Laboratory for Bio-resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, PR China.
Repeated homoploid hybrid speciation (HHS) events with the same parental species have rarely been reported. In this study, we used population transcriptome data to test paraphyly and HHS events in the conifer Picea brachytyla. Our analyses revealed non-sister relationships for two lineages of P.
View Article and Find Full Text PDFPlants (Basel)
November 2022
State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.
Genome-wide single nucleotide polymorphism (SNP) markers were obtained by genotyping-by-sequencing (GBS) technology to study the genetic relationships, population structure, gene flow and selective sweeps during species differentiation of , and from a genome-wide perspective. We used and as outgroups, and three evolutionary branches were obtained: was located on one branch, two populations were grouped onto a second branch, and two populations were grouped onto a third branch. The relationship of with was closer than that with .
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!