Paraburkholderia panacihumi sp. nov., an isolate from ginseng-cultivated soil, is antagonistic against root rot fungal pathogen.

The novel species DCY115 was isolated from ginseng-cultivated soil in Gochang province, Republic of Korea. The isolated strain was assigned to the genus Paraburkholderia due to its 16S rRNA gene sequence proximity to Paraburkholderia xenovorans LB400 (98.8%), Paraburkholderia terricola LMG 20594 (98.4%), Paraburkholderia graminis C4D1M (98.2%), Paraburkholderia rhynchosiae WSM3937 (98.1%), and Paraburkholderia phytofirmans PsJN (98.1%). Strain DCY115 is gram-negative, facultative aerobic, rod-shaped, non-motile, non-flagellated, and oxidase and catalase positive. The predominant isoprenoid quinone of DCY115 is ubiquinone Q-8. The major cellular fatty acids are C, cyclo-C, cyclo-C ω8c, summed feature 3 (C ω7c and/or C ω6c) and summed feature 8 (C ω7c and/or C ω6c). The major polar lipids include diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), and an unknown amino lipid (AL1). The genomic DNA G + C content is 61.3 mol%. Phenotypic tests and chemotaxonomic analysis place strain DCY115 in the genus Paraburkholderia. DNA-DNA hybridization values between strain DCY115 and closely related reference strains were lower than 51%. The low DNA relatedness data in combination with phylogenetic and biochemical tests showed that strain DCY115 could not be assigned to any recognized species. Finally, strain DCY115 showed antagonistic activity against Fusarium solani (KACC 44891) and Cylindrocarpon destructans (KACC 44660), which are two root rot fungal pathogens of ginseng. In conclusion, the results in this study support strain DCY115 as a novel species within the genus Paraburkholderia for which the name Paraburkholderia panacihumi is proposed. The type strain is DCY115 (= KCTC 52952 = JCM 32099).