AI Article Synopsis
- Protein cages are natural structures that can be engineered to hold various types of nonnative materials, like small organic and inorganic molecules.
- Many of these protein cages are based on modified virus capsids that have been altered to prevent infection by excluding genetic material but still enable them to assemble properly.
- The study focuses on utilizing P22 virus-like particles (VLPs) to encapsulate protein cargo through a genetic fusion process, allowing the cargo to be included during the assembly of the protein cage.
Article Abstract
Protein cages are ubiquitous in nature and have been manipulated to encapsulate a range of nonnative cargos including organic, inorganic, and small molecules. Many protein cages are derived from virus capsids that have been rendered noninfectious through the preferential production and use of proteins that are solely involved in capsid assembly, but which do not encapsulate genetic material and therefore do not contribute to infectivity. Here, we describe the production of protein cargo(s) encapsulated inside of P22 virus-like particles (VLPs), derived from bacteriophage P22. This is achieved via genetic fusion of the cargo to a scaffolding protein, which becomes encapsulated in the P22 VLP during templated assembly of the protein cage.
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| http://dx.doi.org/10.1007/978-1-4939-7808-3_20 | DOI Listing |
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