Protective Effects of Bifidobacterial Strains Against Toxigenic .

Probiotics might offer an attractive alternative to prevent and control () infection (CDI). Limited information is available on the ability of commercially used bifidobacterial strains to inhibit . This study examined the anti-clostridial effects of JDM301, a widely used commercial probiotic strain in China, and . evaluation revealed a significant reduction in counts when JDM301 was co-cultured with , which was correlated with the significant decrease in clostridial toxin titres (TcdA and TcdB). Furthermore, the cell-free culture supernatants (CFS) of JDM301 inhibited growth and degraded TcdA and TcdB. Notably, the results showed that acid pH promoted the degradation of TcdA by CFS from JDM301. Furthermore, comparative studies among 10 strains were performed, which showed that the inhibitory effect of CFS from JDM301 was similar with the other 8 strains and higher than strain BLY1. However, when it was neutralized, the significant different was lost. When present together, it was suggested that the acid pH induced by probiotics not only played important roles in the growth inhibition against resulting in the reduction of toxins titres, but also directly promoted the degradation of clostridial toxin. studies proved that JDM301 partially relieved damage to tissues caused by and also decreased the number of and toxin levels. In summary, our results demonstrated that the commercial strain, JDM301 could be considered a probiotic able to exert anti-toxin capability and most of the CFS from were able to inhibit the growth of , depending on acid pH. These results highlighted a potential that JDM301 could be helpful in preventing CDI and that most of the bifidobacterial strains could (at least partially) exert protective effects by reducing toxin titres through growth inhibition against toxigenic .