Poly(C)-Binding Protein Pcbp2 Enables Differentiation of Definitive Erythropoiesis by Directing Functional Splicing of the Runx1 Transcript. | LitMetric
Formation of the mammalian hematopoietic system is under a complex set of developmental controls. Here, we report that mouse embryos lacking the KH domain poly(C) binding protein, Pcbp2, are selectively deficient in the definitive erythroid lineage. Compared to wild-type controls, transcript splicing analysis of the Pcbp2 embryonic liver reveals accentuated exclusion of an exon (exon 6) that encodes a highly conserved transcriptional control segment of the hematopoietic master regulator, Runx1. Embryos rendered homozygous for a Runx1 locus lacking this cassette exon (Runx1ΔE6) effectively phenocopy the loss of the definitive erythroid lineage in Pcbp2 embryos. These data support a model in which enhancement of Runx1 cassette exon 6 inclusion by Pcbp2 serves a critical role in development of hematopoietic progenitors and constitutes a critical step in the developmental pathway of the definitive erythropoietic lineage.
Gastric cancer (GC) is a leading cause of cancer-related deaths worldwide. Poly(rC) binding protein 2 (PCBP2), an RNA binding protein, has been reported to be involved in the progression of various cancers. However, the role of PCBP2 in GC and its underlying mechanisms remain elusive.
Zhejiang Key Laboratory of Precise Protection and Promotion of Fertility, Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, 310016, China.
Massive numbers of modified bases in mRNAs sculpt the epitranscriptome and play vital roles in RNA metabolism. The only known acetylated RNA modification, N-4-acetylcytidine (acC), is highly conserved across cell types and among species. Although the GCN5-related acetyltransferase 10 (NAT10) functions as an acC writer, the mechanism underlying the acetylation process is largely unknown.
* The study found that HNF4A-AS1 is downregulated in HCC tissues and its lower expression correlates with poorer patient prognosis, as it inhibits cancer cell proliferation and invasion.
* Mechanistically, HNF4A-AS1 interacts with PCBP2 to promote its degradation, influencing the stability of AGR2 mRNA and potentially serving as a therapeutic target for HCC.
* Researchers discovered multiple avian calicivirus genomes featuring type 2 IRESs and identified an additional open reading frame (ORF1*) that overlaps with the main ORF1, suggesting complex genomic features in these viruses.
* The study confirmed that ribosomes can initiate translation at multiple sites on the avian calicivirus genomes, and this process requires specific initiation factors, with enhanced initiation occurring thanks to IRES-binding factors that interact with key
Duck hepatitis A virus type 1 (DHAV-1) is an important member of the Picornaviridae family that causes highly fatal hepatitis in ducklings. Since picornaviruses have small genomes with limited coding capacity, they must utilize host proteins for viral cap-independent translation and RNA replication. Here, we report the role of duck poly(rC)-binding protein 2 (PCBP2) in regulating the replication and translation of DHAV-1.
