AI Article Synopsis
- - The study explores using the Mitra microsampler as an alternative to dried blood spot collection for monitoring biopharmaceutical concentrations, particularly therapeutic drugs.
- - Researchers tested various therapeutic monoclonal antibodies in whole blood using both the Mitra microsampler and filter paper, analyzing concentrations with specific ELISAs.
- - Results showed effective recovery rates for several therapeutic antibodies after storage for up to one month, establishing protocols for estimating drug concentrations from capillary blood samples.
Article Abstract
Aim: For assessment of concentrations of biopharmaceuticals, for example, therapeutic drug monitoring, dried blood sampling of capillary blood is a convenient alternative to traditional venepuncture sampling. We investigated an alternative to dried blood spot collection on filter paper: sampling capillary blood using the Mitra microsampler.
Materials And Methods: Therapeutic monoclonal antibodies were spiked in whole blood, sampled using filter paper and Mitra microsampler and concentrations measured using specific ELISAs.
Results: Good recoveries of adalimumab, infliximab, ustekinumab, vedolizumab, tocilizumab, natalizumab and rituximab were found up to 1 month of storage at room temperature, averaging 95.2% for the Mitra microsampler and 92.9% for Whatman paper. Both hemoglobin and potassium yield satisfactory estimates for the volume of the cellular fraction of blood samples in combination with the Mitra microsampler.
Conclusion: We established practical protocols for the estimation of serum/plasma concentrations of therapeutic antibodies via capillary blood microsampling.
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| http://dx.doi.org/10.4155/bio-2018-0010 | DOI Listing |
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