Cellulose type chiral stationary phase based on reduced graphene oxide@silica gel for the enantiomer separation of chiral compounds.

The graphene oxide (GO) was covalently coupled to the surfaces of silica gel (SiO ) microspheres by amide bond to get the graphene oxide@silica gel (GO@SiO ). Then, the GO@SiO was reduced with hydrazine to the reduced graphene oxide@silica gel (rGO@SiO ), and the cellulose derivatives were physically coated on the surfaces of rGO@SiO to prepare a chiral stationary phase (CSP) for high performance liquid chromatography. Under the optimum experimental conditions, eight benzene-enriched enantiomers were separated completely, and the resolution of trans-stilbene oxide perfectly reached 4.83. Compared with the blank column of non-bonded rGO, the separation performance is better on the new CSP, which is due to the existence of rGO to produce special retention interaction with analytes, such as π-π stacking, hydrophobic effect, π-π electron-donor-acceptor interaction, and hydrogen bonding. Therefore, the obtained CSP shows special selectivity for benzene-enriched enantiomers, improves separation selectivity and efficiency, and rGO plays a synergistic effect with cellulose derivatives on enantioseparation.