AI Article Synopsis
- Proteomics research faces challenges due to limited protein sequence databases for non-model species like herbs, but using translated transcriptome databases can help.
- A study utilized SWATH-MS technology alongside transcriptome profiling to analyze the proteome of a tropical herb, marking a first for this type of analysis.
- The research investigated how the phytohormone methyl jasmonate affects protein changes, and the findings were shared in the ProteomeXchange Consortium with the identifier PXD005749.
Article Abstract
Proteomics is often hindered by the lack of protein sequence database particularly for non-model species such as herbs. An integrative approach called proteomics informed by transcriptomics is possible [1], in which translated transcriptome sequence database is used as the protein sequence database. In this current study, the proteome profile were profiled using SWATH-MS technology complemented with documented transcriptome profiling [2], the first such report in this tropical herb. The plant was also elicited using a phytohormone, methyl jasmonate (MeJA) and protein changes were elucidated using label-free quantification of SWATH-MS to understand the role of such signal molecule in this herbal species. The mass spectrometry proteomics data was deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD005749. This data article refers to the article entitled "Proteomics (SWATH-MS)-informed by transcriptomics approach of leaves upon methyl jasmonate elicitation" [3].
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972844 | PMC |
| http://dx.doi.org/10.1016/j.dib.2017.09.063 | DOI Listing |
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