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Proteomic analysis of follicular fluid in carriers and non-carriers of the Trio allele for high ovulation rate in cattle. | LitMetric

AI Article Synopsis

  • - The study aimed to identify differences in follicular fluid proteins between cows that carry and do not carry a specific genetic trait linked to high ovulation rates by using a total of nine cows for initial and validation tests.
  • - Researchers employed a hexapeptide ligand library to enhance the detection of low-abundance proteins during mass spectrometry, leading to the identification of two key proteins (SERPINE2 and INHBB) that showed significant differences between the two groups.
  • - Further analysis revealed three additional proteins with consistent expression differences, suggesting that the high level of SMAD6 in carrier females may hinder a signaling pathway (TGF-β/BMP), which typically promotes protein expression linked to follicular development.

Article Abstract

This study was conducted to characterise differences in follicular fluid proteins between carriers and non-carriers of a bovine allele for high ovulation rate. A total of four non-carrier and five carrier females were used in an initial study with four and six additional non-carriers and carriers respectively used in a validation study. Emergence of the follicular wave was synchronised and the ovaries containing the dominant follicle(s) were extracted by ovariectomy for follicular fluid collection. A hexapeptide ligand library was used to overcome the masking effect of high-abundance proteins and to increase detection of low-abundance proteins in tandem mass spectrometry. After correcting for multiple comparisons, only two proteins, glia-derived nexin precursor (SERPINE2) and inhibin β B chain precursor (INHBB), were significantly differentially expressed (false-discovery rate <0.05). In a replicate study of analogous design differential expression was confirmed (P<0.05). Joint analysis of results from the two studies indicated that three additional proteins were consistently differentially expressed between genotypes. For three of these five, previous studies have indicated that expression is increased by transforming growth factor-β-bone morphogenetic protein signalling; their reduction in follicular fluid from carrier animals is consistent with the ~9-fold overexpression of SMAD family member 6 (SMAD6) in carriers that is inhibitory to this pathway.

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Source
http://dx.doi.org/10.1071/RD17252DOI Listing

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