Enhanced photoelectrochemical DNA sensor based on TiO/Au hybrid structure.

Biosens Bioelectron

Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, School of Chemistry & Chemical Engineering, Anhui University, Hefei 230601, PR China.

Published: September 2018

A novel enhanced photoelectrochemical DNA sensor, based on a TiO/Au hybrid electrode structure, was developed to detect target DNA. The sensor was developed by successively modifying fluorine-tin oxide (FTO) electrodes with TiO nanoparticles, gold (Au) nanoparticles, hairpin DNA (DNA1), and CdSe-COOH quantum dots (QDs), which acted as signal amplification factors. In the absence of target DNA, the incubated DNA1 hairpin and the CdSe-COOH QDs were in close contact with the TiO/Au electrode surface, leading to an enhanced photocurrent intensity due to the sensitization effect. After incubation of the modified electrode with the target DNA, the hairpin DNA changed into a double helix structure, and the CdSe QDs moved away from the TiO/Au electrode surface, leading to a decreased sensitization effect and photoelectrochemical signal intensity. This novel DNA sensor exhibited stable, sensitive and reproducible detection of DNA from 0.1 μM to 10 fM, with a lower detection limit of 3 fM. It provided good specificity, reproducibility, stability and is a promising strategy for the detection of a variety of other DNA targets, for early clinical diagnosis of various diseases.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2018.05.036DOI Listing

Publication Analysis

Top Keywords

dna sensor
16
target dna
12
dna
10
enhanced photoelectrochemical
8
photoelectrochemical dna
8
sensor based
8
based tio/au
8
tio/au hybrid
8
hairpin dna
8
tio/au electrode
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!